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ELISA Kit DIY Materials for Connective Tissue Growth Factor (CTGF)
CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2
- Product No.KSA010Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range31.2-2000pg/mL
- Applicable Sensitivity12.2pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1231
US$ 2052
US$ 3694
US$ 7182
US$ 12312
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF). No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Journal of Orthopaedic Research | Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis model PubMed: 19824055 |
| World Journal of Gastroenterology | Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function PubMed: PMC2726461 |
| PLos One | Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound Healing Plosone: 0032128 |
| Clinical Biochemistry | Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathy ScienceDirect: S0009912012000987 |
| The Journal of Heart and Lung Transplantation | Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development? PubMed: S1053249812010121 |
| Biomedical and Environmental Sciences | The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats Besjournal: Source |
| Disease Markers | Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosis PubMed: 22846210 |
| World Journal of Gastroenterology | Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosis PubMed: PMC3351780 |
| Journal of ethnopharmacology | Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. Pubmed: 24211397 |
| Radiation Oncology | Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapy Ro-journal: Source |
| PLoS One. | Knock Out of S1P3 Receptor Signaling Attenuates Inflammation and Fibrosis in Bleomycin-Induced Lung Injury Mice Model Pubmed:Pmc4157792 |
| European Journal of Heart Failure | Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal models Pubmed:24464932 |
| Bone | Connective Tissue Growth Factor is a Target of Notch Signaling in Cells of the Osteoblastic Lineage Pubmed:24792956 |
| American Journal of Pathology | Disruption of collagen homeostasis can reverse established age-related myocardial fibrosis Pubmed:25701883 |
| J Gastroenterol | Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitis PubMed: 26190501 |
| Life Sciences | Suppression of connective tissue growth factor mediates the renoprotective effect of Sitagliptin rather than Pioglitazone in type 2 diabetes mellitus Pubmed:27049870 |
| International Journal of Molecular Sciences | TGF-β and Physiological Root Resorption of Deciduous Teeth. pubmed:28035998 |
| Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy pubmed:27516211 |
| Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR pubmed:28004175 |
| British Journal of Ophthalmology | The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidence pubmed:27531356 |
| Pathophysiology | Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseases pubmed:27686729 |
| Evidence-Based Complementary and Alternative Medicine | Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway pubmed:28698735 |
| Heart and Vessels | Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR 10.1007/s00380-016-0930-y |
| Cytokine | 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. pubmed:28460256 |
| Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. pubmed:29120858 |
| Life Sciences | Sustained β-AR stimulation induces synthesis and secretion of growth factors in cardiac myocytes that affect on cardiac fibroblast activation Pubmed:29107793 |
| International Journal of Psychophysiology | Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller Cells Pubmed:30123889 |
| Journal of Cellular Physiology | TGFβ mediates collagen production in human CRSsNP nasal mucosa‐derived fibroblasts through Smad2/3‐dependent pathway and CTGF induction and secretion Pubmed: 30426494 |
| Journal of Clinical Medicine | Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with … Pubmed: 30413105 |
| Nature Communications | Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin M Pubmed: 31249305 |
| Cardiology | Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart Failure Pubmed: 31466059 |
| Stem Cells International | CTGF Attenuates Tendon-Derived Stem/Progenitor Cell Aging Pubmed: 31827530 |
| Pharmaceuticals | Small-Dose Sunitinib Modulates p53, Bcl-2, STAT3, and ERK1/2 Pathways and Protects against Adenine-Induced Nephrotoxicity Pubmed: 33212804 |
| BMC Veterinary Research | Lysophosphatidic acid as a regulator of endometrial connective tissue growth factor and prostaglandin secretion during estrous cycle and endometrosis in the … Pubmed: 32943074 |
| Revista Portuguesa de Cardiologia | MicroRNAs and ventricular remodeling in aortic stenosis Pubmed: 32682570 |
| Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction | |
| Exp Gerontol | High carbohydrate high fat diet causes arterial hypertension and histological changes in the aortic wall in aged rats: The involvement of connective tissue growth … 34455071 |
| Research Square | Hepatocyte-specific Smad4 deficiency alleviates liver fibrosis via the p38/p65 pathway |