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ELISA Kit DIY Materials for Angiotensin II (AngII)
Ang-II; Angiotensin-2
- Product No.KSA005Ra11
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range24.69-2000pg/mL
- Applicable Sensitivity9.22pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1231
US$ 2052
US$ 3694
US$ 7182
US$ 12312
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Angiotensin II (AngII). No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Nature Medicine | Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition MolMed: 10_11_liu |
| The European Journal of Clinical Investigation | Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes Wiley: source |
| Immunopharmacology and Inflammation | Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid ScienceDirect: S0188440910001049 |
| Nephrology | Effect of aggregated immunoglobulin A1 from immunoglobulin A nephropathy patients on nephrin expression in podocytes Wiley: source |
| PLoS One | Angiotensin II and Angiotensin-(1-7) in Paraventricular Nucleus Modulate Cardiac Sympathetic Afferent Reflex in Renovascular Hypertensive Rats PubMed: PMC3527547 |
| BioMed Research International | Heterogeneous Downregulation of Angiotensin II AT1-A and AT1-B Receptors in Arterioles in STZ-Induced Diabetic Rat Kidneys Hindawi: 947506 |
| 34 | Biglcan is beneficial in angiotensin II induced heart failure by preventing cardiac inflammation and remodeling improving LV function and mortality by preventing transdifferentiation of myofibroblasts Oxfordjournals: Source |
| Pflügers Archiv-European Journal of Physiology | SOD1 gene transfer into paraventricular nucleus attenuates hypertension and sympathetic activity in spontaneously hypertensive rats Pubmed: 23114721 |
| Eur J Pharmacol. | Angiotensin II and vasopressin are involved in the defense system against anaphylactic hypotension in anesthetized rats Pubmed:24650734 |
| J Mol Cell Cardiol. | Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction Pubmed:25871829 |
| Mol Med Rep. | High glucose induces activation of the local renin?angiotensin system in glomerular endothelial cells Pubmed:24337709 |
| Mol Med Rep. | Angiotensin II promotes the progression of human gastric cancer Pubmed:24424956 |
| PLOS ONE | Intermedin in the Paraventricular Nucleus Attenuates Cardiac Sympathetic Afferent Reflex in Chronic Heart Failure Rats Pubmed:24709972 |
| Journal of Vascular Surgery | Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice Pubmed:25037606 |
| Biochim Biophys Acta. | Cathepsin G activity lowers plasma LDL and reduces atherosclerosis Pubmed:25092171 |
| Acta Physiologica | Superoxide anions in the paraventricular nucleus mediate cardiac sympathetic afferent reflex in insulin resistance rats Pubmed:25307720 |
| Neuroscience Letters | Administration of exogenous 1,25(OH)2D3 normalizes overactivation of the central renin-angiotensin system in 1α(OH)ase knockout mice Pubmed:25576706 |
| 34 | A background Ca2+ entry pathway mediated by TRPC1/TRPC4 is critical for development of pathological cardiac remodelling PubMed: 26069213 |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca2 PubMed: 26706290 |
| Physiological Research | Renal Sympathetic Denervation Improves Cardiac Dysfunction PubMed: 25804096 |
| Nutrition | Effects of Rubus occidentalis extract on blood pressure in subjects with prehypertension: randomized, double-blinded, placebo controlled clinical trial PubMed: 26740254 |
| J Am Soc Hypertens | Angiotensin-(1–7) enhances the effects of angiotensin II on the cardiac sympathetic afferent reflex and sympathetic activity in rostral ventrolateral medulla in renovascular hypertensive rats PubMed: 26428223 |
| PLoS One. | Up ReCavia (Guinea pig )lation of cystathione γ lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol–Caffeine Induced Left Ventricular Hypertrophy in Wistar Kyoto Rats Pubmed:26963622 |
| Nutrition | Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trial Pubmed:26740254 |
| Journal of Atherosclerosis and Thrombosis | Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase ReCavia (Guinea pig )lation in Human Carotid Atheroma in vitro Pubmed:26947595 |
| Mol Neurobiol | A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats Pubmed:26867655 |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro Pubmed:26706290 |
| PLoS One. | Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. pubmed:27880816 |
| Oxidative Medicine and Cellular Longevity | Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Mice pubmed:27698952 |
| Journal of the Renin-Angiotensin-Aldosterone System | Angiotensin type 2 receptor null mice express reduced levels of renal angiotensin converting enzyme-2/angiotensin (1-7)/Mas receptor and exhibit greater high-fat diet-induced kidney injury 1470320316661871.abstract |
| Microvascular Research | Pleiotropic effects of survivin in vascular endothelial cells. pubmed:27373518 |
| PLOS ONE | Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. pubmed:27391973 |
| journal of pharmacological sciences | β-Aminoisobutyric acid ameliorates the renal fibrosis in mouse obstructed kidneys via inhibitionof renal fibroblast activation and fibrosis. pubmed:28433566 |
| Experimental Animals | Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. pubmed:28367863 |
| Oncotarget | Effects of renal denervation on monocrotaline induced pulmonary remodeling pubmed:28187460 |
| The American Journal of Pathology | The Renin-Angiotensin System Regulates Neurodegeneration in a Mouse Model of Optic Neuritis pubmed:28919108 |
| PLoS One | Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy pubmed:29240788 |
| Frontiers in Physiology | Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression pubmed:29066981 |
| Neurotherapeutics | Brain Renin–Angiotensin System Blockade Attenuates Methamphetamine-Induced Hyperlocomotion and Neurotoxicity Pubmed:29464572 |
| International Journal of Cardiology | ALDH2 protects against alcoholic cardiomyopathy through a mechanism involving the p38 MAPK/CREB pathway and local renin-angiotensin system inhibition in … Pubmed:29506687 |
| Neuroscience | Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats Pubmed:29684507 |
| American Journal of Physiology-Renal Physiology | Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats Pubmed:29846109 |
| Journal of the American Society of Hypertension | Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system Pubmed:29752040 |
| Evidence-based Complementary and Alternative Medicine | Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects Pubmed:29977313 |
| Brazilian Journal of Medical and Biological Research | Renovascular hypertension increases serum TNF and CX3CL1 in experimental Trypanosoma cruzi infection Pubmed:29590257 |
| American Journal of Physiology-Heart and Circulatory Physiology | Sex Differences in the Central and Peripheral Manifestations of Ischemia-induced Heart Failure in Rats Pubmed: 30289294 |
| Toxicology Mechanisms and Methods | Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats Pubmed: 30426860 |
| Journal of Clinical and Aesthetic Dermatology | Detection of Angiotensin II and AT1 Receptor Concentrations in Keloid and Hypertrophic Scar. |
| International Journal of Ophthalmology | Serum microRNA-221 as a biomarker for diabetic retinopathy in patients associated with type 2 diabetes Pubmed: 30588418 |
| JOURNAL OF STRENGTH AND CONDITIONING RESEARCH | ACE Gene Plays a Key Role in Reducing Blood Pressure in The Hyperintensive Elderly After Resistance Training Pubmed: 30908459 |
| The American Journal of the Medical Sciences | T lymphocyte-based renin angiotensin system in obesity |
| Journal of Alzheimers Disease | Cerebrospinal Fluid Changes in the Renin-Angiotensin System in Alzheimer's Disease Pubmed: 31594235 |
| Andrologia | Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status Pubmed: 31989676 |
| Molecular Medicine Reports | BPA disrupts 17‑estradiol‑mediated hepatic protection against ischemia/reperfusion injury in rat liver by upregulating the Ang II/AT1R signaling pathway Pubmed: 32319667 |
| MOLECULAR MEDICINE REPORTS | The sympathetic transmitter norepinephrine inhibits VSMC proliferation induced by TGFβ by suppressing the expression of the TGFβ receptor ALK5 in aorta … Pubmed: 32319652 |
| Cell Mol Neurobiol | Predator Scent-Induced Sensitization of Hypertension and Anxiety-like Behaviors Pubmed: 33201417 |
| biochemistry biophysical reports | Effect of repetitive potassium iodide on thyroid and cardiovascular functions in elderly rats Pubmed: 33024842 |
| bioscience reports | Angiotensin II enhances the proliferation of Natural Killer/T-cell lymphoma cells via activating PI3K/Akt signaling pathway Pubmed: 32969473 |
| AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | A (pro) renin receptor decoy peptide PRO20 protects against adriamycin-induced nephropathy by targeting intrarenal renin-angiotensin system Pubmed: 32865014 |
| Acta Endocrinologica-Bucharest | THE ROLE OF HIGH FOLLICULAR LEVELS OF ANGIOTENSIN II AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN ANTICIPATING THE … Pubmed: 32685035 |
| JOURNAL OF PROTEOME RESEARCH | Proteomics and informatics for understanding phases and identifying biomarkers in COVID-19 disease Pubmed: 32657586 |
| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats Pubmed: 32616735 |
| BIOMEDICINE & PHARMACOTHERAPY | Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive rats Pubmed: 32570115 |
| CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats Pubmed: 32349626 |
| Evidence-Based Complementary and Alternative Medicine | Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats Pubmed: 33133215 |
| Oxidative Medicine and Cellular Longevity | Sinapic Acid Attenuates Cardiovascular Disorders in Rats by Modulating Reactive Oxygen Species and Angiotensin Receptor Expression Pubmed: 32765804 |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats |
| Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum … | |
| Physiol Behav | Low-load resistance training with blood flow restriction prevent renal function decline: The role of the redox balance, angiotensin 1-7 and vasopressin 33340514 |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice 33303990 |
| Int J Hypertens | The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models 33884205 |
| Physiol Rep | (Pro) renin receptor antagonist PRO20 attenuates nephrectomy©\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/¦Â©\catenin signaling 34057312 |
| Redox Biology | Renovascular effects of inorganic nitrate following ischemia-reperfusion of the kidney 33360353 |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease 33995926 |
| researchsquare | Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice |
| Molecular Medicine Reports | Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats 34278476 |
| BMC Musculoskeletal Disorders | Ang-(1–7) protects skeletal muscle function in aged mice 34548056 |
| J Am Heart Assoc | Maternal Angiotensin II–Induced Hypertension Sensitizes Postweaning High‐Fat Diet–Elicited Hypertensive Response Through Increased Brain Reactivity in Rat … 34482712 |
| Biomed Pharmacother | Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti … 34736077 |
| J Am Heart Assoc | Loss of the Protective Effect of Estrogen Contributes to Maternal Gestational Hypertension‐Induced Hypertensive Response Sensitization Elicited by Postweaning … Pubmed:35014859 |
| J Gerontol A Biol Sci Med Sci | Dysregulation of ACE-1 in normal aging and the early stages of Alzheimer's disease Pubmed:35396835 |
| Biomedicine & Pharmacotherapy | Corynoline protects ang II-induced hypertensive heart failure by increasing PPARα and Inhibiting NF-κB pathway Pubmed:35658238 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| MAA005Ra21 | Monoclonal Antibody to Angiotensin II (AngII) | WB; IHC; ICC; IP. |
| CEA005Ra | ELISA Kit for Angiotensin II (AngII) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA005Ra | Multiplex Assay Kit for Angiotensin II (AngII) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA005Ra11 | ELISA Kit DIY Materials for Angiotensin II (AngII) | Main materials for "Do It (ELISA Kit) Yourself". |