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Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human SFRP4 Polyclonal Antibody (Catalog FAF878Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Anti-Secreted Frizzled Related Protein 4 (SFRP4) Polyclonal Antibody
FRP-4; FRPHE; Frizzled protein, human endometrium
- Product No.FAF878Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)n/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFlow cytometry.
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
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For more details, please contact local distributors!
USAGE
Flow cytometry: 20µg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| Endocrine Journal | Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerance PubMed: 26041556 |
| Horm Metab Res. | Secreted Frizzled-Related Protein 4 (SFRP4) is Elevated in Patients with Diabetes Mellitus Pubmed:26882051 |
| Obesity (Silver Spring). | Reduced expression of chemerin in visceral adipose tissue associates with hepatic steatosis in patients with obesity pubmed:27768254 |
| Obesity | Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans Pubmed:29427376 |
| Novel marker for gestational diabetes patent:US20180059122A1 | |
| British Journal Of Nutrition | A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile Pubmed: 30375290 |
| American Journal of Cancer Research | SFRP4 is a prognostic marker and correlated with Treg cell infiltration in pancreatic ductal adenocarcinoma Pubmed: 30906634 |
| Diabetologia | Effects of long-term exercise on plasma adipokine levels and inflammation-related gene expression in subcutaneous adipose tissue in sedentary dysglycaemic … Pubmed: 31011777 |
| PLoS One | First trimester secreted Frizzled-Related Protein 4 and other adipokine serum concentrations in women developing gestational diabetes mellitus Pubmed: 33206702 |
| Gastric Cancer | SFRP4 drives invasion in gastric cancer and is an early predictor of recurrence 33277667 |
| Diabetology | Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop … |