≡

Active T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)

VSIG9; VSTM3; V-Set And Immunoglobulin Domain Containing 9; V-Set And Transmembrane Domain Containing 3

ACTIVITY TEST

T cell immunoreceptor with Ig and ITIM domains (TIGIT) is an inhibitory receptor expressed on several types of lymphocytes. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs. Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif. A 170 aa variant diverges after aa 166. Lymphocyte Activation Gene 3 (LAG3) and TIGIT are two potential targets for cancer immunotherapy, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TIGIT and recombinant human LAG3. Briefly, biotin-linked TIGIT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LAG3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human TIGIT and recombinant human LAG3 was shown in Figure 1, the EC50 for this effect is 0.11 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APN056Hu61 Active T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) Cell culture; Activity Assays.
RPN056Hu01 Recombinant T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) Positive Control; Immunogen; SDS-PAGE; WB.
RPN056Hu02 Recombinant T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) Positive Control; Immunogen; SDS-PAGE; WB.
EPN056Hu61 Eukaryotic T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) Positive Control; Immunogen; SDS-PAGE; WB.
PAN056Hu01 Polyclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) WB
LAN056Hu81 FITC-Linked Polyclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) WB; IHC; ICC; IF.
LAN056Hu71 Biotin-Linked Polyclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) WB; IHC; ICC.
MAN056Hu22 Monoclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) WB; IHC; ICC; IP.
MAN056Hu23 Monoclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) WB; IHC; ICC; IP.
MAN056Hu24 Monoclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) WB; IHC; ICC; IP.
MAN056Hu21 Monoclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) WB
MAN056Hu25 Monoclonal Antibody to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) WB; IHC; ICC; IP.
SEN056Hu ELISA Kit for T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) Enzyme-linked immunosorbent assay for Antigen Detection.
LMN056Hu Multiplex Assay Kit for T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.