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Active Semaphorin 3A (SEMA3A)

SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A

ACTIVITY TEST

The Semaphorin 3A (SEMA3A) which belongs to the semaphorin family can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Semaphorin 3A is secreted protein containing a Sema domain, an immunoglobulin C2-like domain and a basic domain near the carboxyl tail. It can be secreted by neurons and surrounding tissue to guide migrating cells and axons in the developing nervous system. Besides, Neuropilin 1 (NRP1) has been identified as an interactor of SEMA3A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SEMA3A and recombinant human NRP1. Briefly, SEMA3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to NRP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SEMA3A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SEMA3A and NRP1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Arthritis Research & Therapy Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus Biomed: ar3881
journal of invastigative dermatology Cathelicidin LL-37 Induces Semaphorin 3A Expression in Human Epidermal Keratinocytes: Implications for Possible Application to Pruritus PubMed: 26121211
Neurology Neurofilament light chain level is a weak risk factor for the development of MS. pubmed:27521440
Development miR-126-5p promotes retinal endothelial cell survival through SetD5 regulatio in neurons pubmed:29180574
A Preliminary Study of the Effect of Semaphorin 3A and Acitretin on the Proliferation, Migration, and Apoptosis of HaCaT Cells
Life Sciences Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway. Pubmed: 32603817
ACS NANO Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling Pubmed: 32413258
PLoS One Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes 33905439
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APL917Hu02 Active Semaphorin 3A (SEMA3A) Cell culture; Activity Assays.
APL917Hu01 Active Semaphorin 3A (SEMA3A) Cell culture; Activity Assays.
RPL917Hu01 Recombinant Semaphorin 3A (SEMA3A) Positive Control; Immunogen; SDS-PAGE; WB.
RPL917Hu02 Recombinant Semaphorin 3A (SEMA3A) Positive Control; Immunogen; SDS-PAGE; WB.
PAL917Hu01 Polyclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC; IP.
PAL917Hu02 Polyclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC; IP.
LAL917Hu71 Biotin-Linked Polyclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC.
MAL917Hu22 Monoclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC; IP.
MAL917Hu23 Monoclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC; IP.
MAL917Hu24 Monoclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC; IP.
MAL917Hu21 Monoclonal Antibody to Semaphorin 3A (SEMA3A) WB,IHC
LAL917Hu72 Biotin-Linked Monoclonal Antibody to Semaphorin 3A (SEMA3A) WB; IHC; ICC.
SEL917Hu ELISA Kit for Semaphorin 3A (SEMA3A) Enzyme-linked immunosorbent assay for Antigen Detection.
SCL917Hu CLIA Kit for Semaphorin 3A (SEMA3A) Chemiluminescent immunoassay for Antigen Detection.
LML917Hu Multiplex Assay Kit for Semaphorin 3A (SEMA3A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.