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Active Granzyme H (GZMH)

CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2; Cathepsin G-Like 2,Protein h-CCPX; Cytotoxic T-lymphocyte proteinase; Cytotoxic serine protease C

ACTIVITY TEST

Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme H’s functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type. Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1-18), a propeptide (residues 19-20) and a mature chain (residues 21-246). The purified recombinant human Granzyme H consists of residues 19 to 246 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (Assay Buffer), initiated by addition 50 μL of various concentrations of GZMH (diluted by Assay Buffer) to 50 µL of 1.2 mM Substrate and DTNB mixture. The final well serves as a negative control with no GZMH, replaced with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme H is >300 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPL575Hu01 Recombinant Granzyme H (GZMH) Positive Control; Immunogen; SDS-PAGE; WB.
APL575Hu01 Active Granzyme H (GZMH) Cell culture; Activity Assays.
PAL575Hu01 Polyclonal Antibody to Granzyme H (GZMH) WB; IHC; ICC; IP.
MAL575Hu21 Monoclonal Antibody to Granzyme H (GZMH) WB; IHC; ICC; IP.
SEL575Hu ELISA Kit for Granzyme H (GZMH) Enzyme-linked immunosorbent assay for Antigen Detection.
LML575Hu Multiplex Assay Kit for Granzyme H (GZMH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.