Packages (Simulation)
Image (II)
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Figure. SDS-PAGE
Quality Guarantee
Certificate
Active Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1)
ZA2G; ZAG
- Product No.APL231Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point6.6
- ApplicationsCell culture; Activity Assays.
- Downloadn/a
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 272
US$ 680
US$ 1360
US$ 4080
US$ 10200
For more details, please contact local distributors!
ACTIVITY TEST
Alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1), also known as zinc-α2-glycoprotein (ZAG), is a soluble protein belonging to the major histocompatibility complex (MHC) class I family. It is widely expressed in various bodily fluids and epithelial tissues. First identified in human plasma, AZGP1 exhibits multifunctional properties—most notably regulating lipid metabolism by stimulating lipolysis in adipocytes. As an important adipokine, it promotes fat breakdown and weight loss. Besides,Retinol Binding Protein 4 (RBP4) has been identified as an interactor of LOX, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human LOX and recombinant bovine RBP4. Briefly, LOX was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RBP4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LOX pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human LOX and recombinant bovine RBP4 was shown in Figure 1, the EC50 for this effect is 1.06 µg/mL..
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Journal of Proteomics | Identification of plasma Complement C3 as a potential biomarker for neuroblastoma using a quantitative proteomic approach ScienceDirect: S1874391913005472 |
| International journal of endocrinology | Serum ZAG Levels Were Associated with eGFR Mild Decrease in T2DM Patients with Diabetic Nephropathy. pubmed:28352283 |
| Quantitative Proteomic Analyses of Tear Fluid in Graves' Orbitopathy ISBN 978-82-8377-114-5 | |
| Frontiers in Endocrinology | Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … Pubmed:29755407 |
| International Journal of Endocrinology | Serum Zinc-α2-Glycoprotein Levels in Patients with or without Coronary Artery Disease in Chinese North Population Pubmed: 32184822 |
| Frontiers in Endocrinology | Low Serum ZAG Levels Correlate With Determinants of the Metabolic Syndrome in Chinese Subjects Pubmed: 32265843 |
| Diabetes Metab Syndr Obes | Serum ZAG and Adiponectin Levels Were Closely Related to Obesity and the Metabolically Abnormal Phenotype in Chinese Population Pubmed: 32904655 |
| J Endocrinol Invest | A study on serum pro-neurotensin (PNT), furin, and zinc alpha-2-glycoprotein (ZAG) levels in patients with acromegaly Pubmed:35670958 |