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Active Lysine Specific Demethylase 1A (KDM1A)

AOF2; KDM1; BHC110; LSD1; Amine Oxidase Flavin Containing Domain 2; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2

ACTIVITY TEST

Lysine-specific histone demethylase 1A（LSD1）, also known as KDM1A (lysine-specific demethylase 1A) or AOF2 (flavin-containing amine oxidase domain-containing protein 2), is a type of histone modification enzyme.It is known to specifically remove methyl groups from lysine residues on histone proteins, thereby regulating chromatin structure and gene expression. LSD1 plays a critical role in various biological processes, including cell cycle progression, apoptosis, and tumorigenesis.RCOR1, LSD1, and HDAC1 can form stable, enzymatically active, stoichiometric ternary complexe.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human LSD1 and recombinant human HDAC1. Briefly, biotin-linked LSD1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to HDAC1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human LSD1 and recombinant human HDAC1 was shown in Figure 1, the EC50 for this effect is 4.35ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APG802Hu01 Active Lysine Specific Demethylase 1A (KDM1A) Cell culture; Activity Assays.
RPG802Hu01 Recombinant Lysine Specific Demethylase 1A (KDM1A) Positive Control; Immunogen; SDS-PAGE; WB.