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Active Peptidyl Arginine Deiminase Type IV (PADI4)
PAD; PADI5; PDI4; PDI5; Peptidyl Arginine Deiminase Type V; Peptidylarginine deiminase IV
- Product No.APF709Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point6.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
ACTIVITY TEST
Peptidylarginine deiminase 4 (PADI4) is an enzyme that plays an important role in the organism. It mainly catalyzes the conversion of arginine residues in proteins into citrulline residues. PADI4 plays a key role in various physiological processes such as cell differentiation and immune response. The abnormal expression or change in its activity is closely related to autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosus, making it an important target in the research of these related diseases.The activity of recombinant human PADI4 is determined by its ability to catalyze Benzoyl-arginine ethyl ester (BAEE) to form citrulline.The reaction was performed in 50 mM Tris, 0.1 M NaCl, 10 mM CaCl2, 5 mM DTT solution (Assay Buffer, pH 7.5). A total volume of 300 μL was achieved by adding 75 μL of 100 μg/mL PADI4 to 75 μL of 10 mM BAEE. After 30-minute incubation at 37°C, the reaction mixture was acidified with color-developing solution containing ammonium iron sulfate and measured at 540 nm .
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Arthritis Res Ther. | Enhanced neutrophil extracellular trap generation in rheumatoid arthritis: analysis of underlying signal transduction pathways and potential diagnostic utility Pubmed:Pmc4229860 |
| Journal of Molecular Cell Biology | Peptidylarginine deiminase 4 concentration, but not polymorphisms, is associated with ICU mortality in septic shock patients Pubmed:30044533 |
| Journal of Inflammation Research | Release of active peptidylarginine deiminase into the circulation during acute inflammation induced by coronary artery bypass surgery Pubmed: 31213874 |
| Revista da Associacao Medica Brasileira | Evaluation of peptidylarginine deiminase 4 and PADI4 polymorphisms in sepsis-induced acute kidney injury |