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Active Histone Deacetylase 6 (HDAC6)

HD6; JM21

ACTIVITY TEST

Histone Deacetylase 6 (HDAC6) is an enzyme that belongs to the histone deacetylase family. It is responsible for removing acetyl groups from lysine residues in histone proteins, thereby playing a crucial role in regulating gene expression through chromatin remodeling. HDAC6 is unique among HDACs in that it is primarily localized in the cytoplasm and is involved in a variety of cellular processes, including protein degradation, cell motility, and autophagy.Besides,ANuclear Receptor Corepressor 1 (NCOR1) has been identified as an interactor of HDAC6, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse HDAC6 and recombinant human NCOR1. Briefly, HDAC6 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NCOR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HDAC6 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat HDAC6 and recombinant human NCOR1 was shown in Figure 1, the EC50 for this effect is 0.01ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLOS ONE Dynein Function and Protein Clearance Changes in Tumor Cells Induced by a Kunitz-Type Molecule, Amblyomin-X Pubmed:25479096
disease markers Upregulated Expression of Intestinal Antimicrobial Peptide HD5 Associated with Renal Function in IgA Nephropathy Pubmed: 32089753
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE HDAC6‐mediated α‐tubulin deacetylation suppresses autophagy and enhances motility of podocytes in diabetic nephropathy Pubmed: 32885602
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