≡

Active Sphingosine 1 Phosphate Lyase 1 (SGPL1)

SPL; S1PL; Sphingosine-1-phosphate aldolase

ACTIVITY TEST

Sphingosine 1 Phosphate Lyase 1 (SGPL1) is an enzyme that plays a crucial role in lipid metabolism. It specifically catalyzes the cleavage of sphingosine-1-phosphate (S1P) into phosphoethanolamine and hexadecenal, regulating the levels of S1P in cells. S1P is a bioactive sphingolipid that acts as a signaling molecule, influencing various cellular processes such as cell migration, proliferation, survival, and differentiation. By modulating S1P levels, SGPL1 plays a vital role in maintaining cellular homeostasis and participating in various physiological and pathological processes.Besides,Ephrin A5 (EFNA5) has been identified as an interactor of SGPL1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SGPL1 and recombinant human EFNA5. Briefly, SGPL1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EFNA5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SGPL1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human SGPL1 and recombinant human EFNA5 was shown in Figure 1, the EC50 for this effect is 1.15ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE691Hu01 Recombinant Sphingosine 1 Phosphate Lyase 1 (SGPL1) Positive Control; Immunogen; SDS-PAGE; WB.
APE691Hu01 Active Sphingosine 1 Phosphate Lyase 1 (SGPL1) Cell culture; Activity Assays.
PAE691Hu01 Polyclonal Antibody to Sphingosine 1 Phosphate Lyase 1 (SGPL1) WB; IHC