≡

Active Proenkephalin (PENK)

Preproenkephalin; Proenkephalin-A; Synenkephalin; Met-enkephalin; Met-enkephalin-Arg-Gly-Leu; Leu-enkephalin; Met-enkephalin-Arg-Phe

ACTIVITY TEST

Proenkephalin (PENK) is a protein encoded by the PENK gene. It is initially synthesized as a preproprotein and undergoes proteolytic processing to generate multiple products. As PENK has the function of inhibit the migration cells，we measure the activity of recombinant mouse PENK by the ability of the protein to inhibit the migration of U2OS cells. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the inhibition of chemotactic effect of recombinant mouse PENK on U2OS cell line. Briefly, U2OS cells were seeded into the upper chambers and PENK was added in lower chamber with a polycarbonate filter (8 μm pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×10) and the number of migrated cells were counted randomly (five fields for each filter). Result shows MIP-3α is able to induce migration of U2OS cells. The migrated U2OS cells in low chamber at low magnification (×10) were shown in Figure 1.

USAGE

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APD396Mu01 Active Proenkephalin (PENK) Cell culture; Activity Assays.
RPD396Mu02 Recombinant Proenkephalin (PENK) Positive Control; Immunogen; SDS-PAGE; WB.
RPD396Mu01 Recombinant Proenkephalin (PENK) Positive Control; Immunogen; SDS-PAGE; WB.
PAD396Mu01 Polyclonal Antibody to Proenkephalin (PENK) WB; IHC; ICC; IP.
PAD396Mu02 Polyclonal Antibody to Proenkephalin (PENK) WB; IHC; ICC; IP.
SED396Mu ELISA Kit for Proenkephalin (PENK) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD396Mu Multiplex Assay Kit for Proenkephalin (PENK) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.