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Active Troponin T Type 1, Slow Skeletal (TNNT1)
ANM; STNT; TNT; TNTS; Slow Skeletal Muscle Troponin T
- Product No.APD231Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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ACTIVITY TEST
Troponin T Type 1, Slow Skeletal (TNNT1) is a protein predominantly expressed in slow-twitch skeletal muscles. It is a crucial component of the troponin complex, which plays a vital role in the regulation of muscle contraction. TNNT1 helps to anchor the troponin complex to tropomyosin on the thin filaments of muscle cells. Mutations in the TNNT1 gene can lead to various myopathies, disrupting normal muscle function. It has a specific structure and function that enables it to interact precisely with other troponin subunits.Besides,it has been identified that interaction between TNNT1 and TNNC2 contributes to allowing TNNC2's calcium-induced change to be relayed, triggering muscle contraction regulation.Thus, a functional binding ELISA assay was used to detect the biological activity of TNNT1Briefly, TNNC2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNNT1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNNC2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. Measured by its binding ability in a functional ELISA. When Recombinant TNNT1 is lmmobilized at 2 ug/mL(100 uLwell), the concentration of TNNC2 that produces 50% optimal bindingresponse is found to be approximately 0.00597 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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