ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1)

ANM; STNT; TNT; TNTS; Slow Skeletal Muscle Troponin T

Specificity

This assay has high sensitivity and excellent specificity for detection of Troponin T Type 1, Slow Skeletal (TNNT1).
No significant cross-reactivity or interference between Troponin T Type 1, Slow Skeletal (TNNT1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Troponin T Type 1, Slow Skeletal (TNNT1) and the recovery rates were calculated by comparing the measured value to the expected amount of Troponin T Type 1, Slow Skeletal (TNNT1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-98 93
EDTA plasma(n=5) 93-104 98
heparin plasma(n=5) 85-92 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin T Type 1, Slow Skeletal (TNNT1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin T Type 1, Slow Skeletal (TNNT1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Troponin T Type 1, Slow Skeletal (TNNT1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-104% 85-103% 82-96% 95-103%
EDTA plasma(n=5) 95-105% 94-102% 92-99% 84-98%
heparin plasma(n=5) 80-91% 97-104% 80-97% 93-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Oncotarget A multimodal assessment of balance in elderly and young adults. pubmed:26934319
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD231Hu01 Recombinant Troponin T Type 1, Slow Skeletal (TNNT1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD231Hu01 Polyclonal Antibody to Troponin T Type 1, Slow Skeletal (TNNT1) WB; IHC; ICC; IP.
FAD231Hu01 Anti-Troponin T Type 1, Slow Skeletal (TNNT1) Polyclonal Antibody Flow cytometry.
LAD231Hu71 Biotin-Linked Polyclonal Antibody to Troponin T Type 1, Slow Skeletal (TNNT1) WB; IHC; ICC.
LAD231Hu81 FITC-Linked Polyclonal Antibody to Troponin T Type 1, Slow Skeletal (TNNT1) WB; IHC; ICC; IF.
MAD231Hu21 Monoclonal Antibody to Troponin T Type 1, Slow Skeletal (TNNT1) WB; IHC; ICC; IP.
SED231Hu ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD231Hu Multiplex Assay Kit for Troponin T Type 1, Slow Skeletal (TNNT1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD231Hu01 ELISA Kit DIY Materials for Troponin T Type 1, Slow Skeletal (TNNT1) Main materials for "Do It(ELISA Kit) Yourself"