≡

Active Cubilin (CUBN)

IFCR; MGA1; gp280; Intrinsic Factor Cobalamin Receptor; 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-vitamin B12 receptor

ACTIVITY TEST

Cubilin (CUBN) is a 460 kDa intrinsic factor-vitamin B12 receptor protein and it is expressed in kidney and small intestine. CUBN is a component of a multiligand endocytic receptor complex, which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake. Besides CUBN serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac and it plays an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CUBN and recombinant mouse APOA1. Briefly, CUBN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APOA1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CUBN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CUBN and recombinant mouse APOA1 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC411Hu02 Recombinant Cubilin (CUBN) Positive Control; Immunogen; SDS-PAGE; WB.
APC411Hu02 Active Cubilin (CUBN) Cell culture; Activity Assays.
RPC411Hu01 Recombinant Cubilin (CUBN) Positive Control; Immunogen; SDS-PAGE; WB.
PAC411Hu01 Polyclonal Antibody to Cubilin (CUBN) WB; IHC; ICC; IP.
PAC411Hu02 Polyclonal Antibody to Cubilin (CUBN) WB; IHC; ICC; IP.
MAC411Hu22 Monoclonal Antibody to Cubilin (CUBN) WB; IHC; ICC; IP.
SEC411Hu ELISA Kit for Cubilin (CUBN) Enzyme-linked immunosorbent assay for Antigen Detection.
AEC411Hu ELISA Kit for Anti-Cubilin Antibody (Anti-CUBN) Enzyme-linked immunosorbent assay for Antibody Detection.
LMC411Hu Multiplex Assay Kit for Cubilin (CUBN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.