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Active Asporin (ASPN)

PLAP1; SLRR1C; Periodontal ligament-associated protein 1

ACTIVITY TEST

Asporin (ASPN) is a protein belongs to a family of leucine-rich repeat (LRR) proteins implicated in cancer, osteoarthritis, and periodontal ligament mineralization. The protein negatively regulates chondrogenesis in the articular cartilage and periodontal ligament (PDL) differentiation, inhibits BMP2-induced cytodifferentiation of PDL cells and also nhibits the interaction between TGFB1 and TGF-beta receptor type II in the presence of heparin/heparan sulfate in vitro. In addition Lumican (LUM) and ASPN can contribute to the fibrinogenesis of cardiac remodeling of ICM, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ASPN and recombinant mouse LUM. Briefly, ASPN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LUM-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ASPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse ASPN and recombinant mouse LUM was shown in Figure 1, the EC50 for this effect is 2.6 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC321Mu02 Recombinant Asporin (ASPN) Positive Control; Immunogen; SDS-PAGE; WB.
APC321Mu01 Active Asporin (ASPN) Cell culture; Activity Assays.
RPC321Mu03 Recombinant Asporin (ASPN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC321Mu04 Recombinant Asporin (ASPN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC321Mu01 Recombinant Asporin (ASPN) Positive Control; Immunogen; SDS-PAGE; WB.
PAC321Mu02 Polyclonal Antibody to Asporin (ASPN) WB; IHC; ICC; IP.
PAC321Mu01 Polyclonal Antibody to Asporin (ASPN) WB; IHC; ICC; IP.
SEC321Mu ELISA Kit for Asporin (ASPN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC321Mu Multiplex Assay Kit for Asporin (ASPN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.