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Active Collagen Type XV (COL15)

COL15A1; COL15-A1; Collagen Alpha-1(XV)chain; Restin; Endostatin-XV; Related to endostatin;

ACTIVITY TEST

Collagen Type XV (COL15, encoded by COL15A1) is a non-fibrillar FACIT collagen, widely distributed in human tissues and highly enriched in basement membrane zones. It forms a homotrimeric structure with interrupted collagenous domains, functioning to anchor basement membranes to underlying connective tissue stroma and stabilize tissue architecture. Its C-terminal fragment, restin, exhibits anti-angiogenic activity. COL15 acts as a tumor suppressor; its loss correlates with muscle/microvessel degeneration and enhanced cancer cell invasion. Hypoxia upregulates COL15 via HIF-1α, and it modulates epithelial-mesenchymal transition (EMT) in tumor microenvironments. COL15 indirectly interacts with E-cadherin by regulating EMT signaling, thereby stabilizing E-cadherin-mediated cell-cell adhesion.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human COL15 and recombinant human EMT. Briefly, COL15 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EMT-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-COL15 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human COL15 and recombinant human EMT was shown in Figure 1, the EC50 for this effect is 0.0117 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.5-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC137Hu01 Recombinant Collagen Type XV (COL15) Positive Control; Immunogen; SDS-PAGE; WB.
APC137Hu01 Active Collagen Type XV (COL15) Cell culture; Activity Assays.
PAC137Hu01 Polyclonal Antibody to Collagen Type XV (COL15) ICC/IF
LAC137Hu71 Biotin-Linked Polyclonal Antibody to Collagen Type XV (COL15) WB; IHC; ICC.
LAC137Hu81 FITC-Linked Polyclonal Antibody to Collagen Type XV (COL15) WB; IHC; ICC; IF.
MAC137Hu22 Monoclonal Antibody to Collagen Type XV (COL15) WB
LAC137Hu72 Biotin-Linked Monoclonal Antibody to Collagen Type XV (COL15) WB; IHC; ICC.
LAC137Hu82 FITC-Linked Monoclonal Antibody to Collagen Type XV (COL15) WB; IHC; ICC; IF.
SEC137Hu ELISA Kit for Collagen Type XV (COL15) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC137Hu Multiplex Assay Kit for Collagen Type XV (COL15) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.