≡

Active Interleukin 4 Receptor (IL4R)

CD124; IL4-R; IL4RA; IL4-BP; Soluble interleukin-4 receptor subunit alpha; IL-4-binding protein

ACTIVITY TEST

Interleukin 4 Receptor (IL4R) is a transmembrane protein that plays a pivotal role in the immune system, particularly in mediating type 2 immune responses. Signaling through IL4R promotes B-cell activation, antibody class switching to IgE, and differentiation of Th2 cells, while also contributing to anti-inflammatory responses and alternative macrophage activation. Dysregulation of IL4R signaling is associated with allergic diseases, asthma, and certain parasitic infections. IL4R binds its cognate ligand Interleukin 4 (IL4) with high affinity, initiating dimerization of receptor subunits and subsequent activation of the JAK-STAT signaling pathway.To detect the activity of recombinant IL4R , a functional ELISA assay was performed to evaluate the interaction between recombinant human IL4R and recombinant rabbit IL4.Briefly, IL4R was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL4R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human IL4R and recombinant rabbit IL4 was shown in Figure 1, the EC50 for this effect is 0.056µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Int J Nanomedicine Treating glioblastoma multiforme with selective high-dose liposomal doxorubicin chemotherapy induced by repeated focused ultrasound PubMed: PMC3289450
Multidisciplinary Respiratory Medicine Role of interleukin 4 and its receptor in clinical presentation of chronic extrinsic allergic alveolitis: a pilot study PubMed: PMC3682858
The Open Respiratory Medicine Journal Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias PubMed: PMC3551240
Exp Ther Med. PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis Pubmed:Pmc4079423
diagnostics Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡­ 33924683
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC031Hu01 Recombinant Interleukin 4 Receptor (IL4R) Positive Control; Immunogen; SDS-PAGE; WB.
APC031Hu01 Active Interleukin 4 Receptor (IL4R) Cell culture; Activity Assays.
PAC031Hu01 Polyclonal Antibody to Interleukin 4 Receptor (IL4R) IHC; ICC/IF
RAC031Hu21 Recombinant Antibody to Interleukin 4 Receptor (IL4R) WB; IF; ICC; IHC; IP; FCM.
MAC031Hu22 Monoclonal Antibody to Interleukin 4 Receptor (IL4R) WB; IHC; ICC; IP.
SEC031Hu ELISA Kit for Interleukin 4 Receptor (IL4R) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC031Hu Multiplex Assay Kit for Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.