≡

Multiplex Assay Kit for Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD124; IL4-R; IL4RA; IL4-BP; Soluble interleukin-4 receptor subunit alpha; IL-4-binding protein

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-101 97
EDTA plasma(n=5) 92-101 95
heparin plasma(n=5) 83-99 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 81-94% 81-95% 92-101%
EDTA plasma(n=5) 79-92% 97-105% 96-105% 92-105%
heparin plasma(n=5) 78-93% 90-97% 82-91% 85-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:IL4R) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Int J Nanomedicine Treating glioblastoma multiforme with selective high-dose liposomal doxorubicin chemotherapy induced by repeated focused ultrasound PubMed: PMC3289450
Multidisciplinary Respiratory Medicine Role of interleukin 4 and its receptor in clinical presentation of chronic extrinsic allergic alveolitis: a pilot study PubMed: PMC3682858
The Open Respiratory Medicine Journal Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias PubMed: PMC3551240
Exp Ther Med. PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis Pubmed:Pmc4079423
diagnostics Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡­ 33924683
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC031Hu01 Recombinant Interleukin 4 Receptor (IL4R) Positive Control; Immunogen; SDS-PAGE; WB.
PAC031Hu01 Polyclonal Antibody to Interleukin 4 Receptor (IL4R) WB; IHC; ICC; IP.
RAC031Hu21 Recombinant Antibody to Interleukin 4 Receptor (IL4R) WB; IF; ICC; IHC; IP; FCM.
MAC031Hu22 Monoclonal Antibody to Interleukin 4 Receptor (IL4R) WB; IHC; ICC; IP.
SEC031Hu ELISA Kit for Interleukin 4 Receptor (IL4R) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC031Hu Multiplex Assay Kit for Interleukin 4 Receptor (IL4R) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.