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Active Complement Factor B (CFB)

CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein

ACTIVITY TEST

Complement factor B (CFB), a 95-kDa protein, is a crucial catalytic element of the alternative pathway (AP) of complement. After binding of CFB to C3b, activation of the AP depends on the proteolytic cleavage of CFB by factor D to generate the C3 convertase (C3bBb). The C3 convertase contains the catalytic subunit of CFB (Bb), the enzymatic site for the cleavage of a new molecule of C3 into C3b. Thus, a binding ELISA assay was conducted to detect the association of recombinant mouse CFB with recombinant mouse CFD. Briefly, biotin-linked recombinant mouse CFB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CFD-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse CFB and recombinant mouse CFD was shown in Figure 1, the EC50 for this effect is 1.238 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
BMC Nephrology Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysis BioMed: 14712369
J. Proteome Res. Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS ACS: pr300330z
Pediatr Int Atypical hemolytic uremic syndrome: Korean pediatric series PubMed: 25443527
Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358
Biochemical and Biophysical Research Communications Adipose tissue complement factor B promotes adipocyte maturation. pubmed:29137982
Journal of proteome research Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection
journal of human genetics Identification of susceptibility locus shared by IgA nephropathy and inflammatory bowel disease in a Chinese Han population Pubmed: 31857673
Nephrol Dial Transplant Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis 33367879
JOURNAL OF GENERAL VIROLOGY Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse 33410734
J Proteome Res Early Diagnostic Ability of Human Complement Factor B in Pancreatic Cancer Is Partly Linked to Its Potential Tumor-Promoting Role 34766501
J Clin Med A Three-Protein Panel to Support the Diagnosis of Sepsis in Children Pubmed:35329889
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC011Mu02 Recombinant Complement Factor B (CFB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC011Mu03 Recombinant Complement Factor B (CFB) Positive Control; Immunogen; SDS-PAGE; WB.
APC011Mu03 Active Complement Factor B (CFB) Cell culture; Activity Assays.
RPC011Mu01 Recombinant Complement Factor B (CFB) Positive Control; Immunogen; SDS-PAGE; WB.
APC011Mu01 Active Complement Factor B (CFB) Cell culture; Activity Assays.
PAC011Mu02 Polyclonal Antibody to Complement Factor B (CFB) WB; IHC; ICC; IP.
PAC011Mu01 Polyclonal Antibody to Complement Factor B (CFB) WB; IHC; ICC; IP.
LAC011Mu71 Biotin-Linked Polyclonal Antibody to Complement Factor B (CFB) WB; IHC; ICC.
LAC011Mu72 Biotin-Linked Monoclonal Antibody to Complement Factor B (CFB) WB; IHC; ICC.
WEC011Mu Wide-range ELISA Kit for Complement Factor B (CFB) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC011Mu ELISA Kit for Complement Factor B (CFB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC011Mu Multiplex Assay Kit for Complement Factor B (CFB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.