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Active Complement Factor B (CFB)

CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein

ACTIVITY TEST

Complement Factor B (CFB) a component of the alternative pathway of complement activation. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease that associates with C3b to form the alternative pathway C3 convertase. The method of functional assay of CFB was tested in hemolysis assays. Two-fold dilute the recombinant human CFB with 0.9% NaCl, 2mmol/L MgCl2 ,and then add same volume of 1% rabbit erythrocyte (RaE) in 8mmol/L EDTA, the negative control only without MgCl2. All the samples incubated at 37℃. After 3 hours later, take 10μL supernatant and 90μL TMB incubated at 37℃ for 5-10 minutes. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The results are shown in Figure 1. It was obvious that the minimal effective concentration of CFB is 0.3125μg/mL.(A) 1% rabbit erythrocyte (RaE) treated with 0.3125μg/mL CFB for 1h
(B) Negative control (1% RaE treated with 0.3125ug/mL CFB, 8mmol/L EDTA) without MgCl2.
Figure. The hemolysis effection of recombinant human CFB

Figure. The hemolysis activity of recombinant human CFB

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
BMC Nephrology Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysis BioMed: 14712369
J. Proteome Res. Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS ACS: pr300330z
Pediatr Int Atypical hemolytic uremic syndrome: Korean pediatric series PubMed: 25443527
Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358
Biochemical and Biophysical Research Communications Adipose tissue complement factor B promotes adipocyte maturation. pubmed:29137982
Journal of proteome research Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection
journal of human genetics Identification of susceptibility locus shared by IgA nephropathy and inflammatory bowel disease in a Chinese Han population Pubmed: 31857673
Nephrol Dial Transplant Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis 33367879
JOURNAL OF GENERAL VIROLOGY Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse 33410734
J Proteome Res Early Diagnostic Ability of Human Complement Factor B in Pancreatic Cancer Is Partly Linked to Its Potential Tumor-Promoting Role 34766501
J Clin Med A Three-Protein Panel to Support the Diagnosis of Sepsis in Children Pubmed:35329889
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC011Hu05 Recombinant Complement Factor B (CFB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC011Hu03 Recombinant Complement Factor B (CFB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC011Hu02 Recombinant Complement Factor B (CFB) Positive Control; Immunogen; SDS-PAGE; WB.
EPC011Hu61 Eukaryotic Complement Factor B (CFB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC011Hu04 Recombinant Complement Factor B (CFB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC011Hu01 Recombinant Complement Factor B (CFB) Positive Control; Immunogen; SDS-PAGE; WB.
APC011Hu03 Active Complement Factor B (CFB) Cell culture; Activity Assays.
PAC011Hu03 Polyclonal Antibody to Complement Factor B (CFB) WB; IHC
PAC011Hu02 Polyclonal Antibody to Complement Factor B (CFB) WB; IHC; ICC; IP.
PAC011Hu01 Polyclonal Antibody to Complement Factor B (CFB) WB; IHC
PAC011Hu04 Polyclonal Antibody to Complement Factor B (CFB) WB; IHC; ICC; IP.
LAC011Hu71 Biotin-Linked Polyclonal Antibody to Complement Factor B (CFB) WB; IHC; ICC.
MAC011Hu22 Monoclonal Antibody to Complement Factor B (CFB) WB; IHC; ICC; IP.
WEC011Hu Wide-range ELISA Kit for Complement Factor B (CFB) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC011Hu ELISA Kit for Complement Factor B (CFB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC011Hu Multiplex Assay Kit for Complement Factor B (CFB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.