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Active Protein S (PROS)

PSA; PS; PS26; PS21; PS22; PS23; PS24; PS25; VKDPS; VKDP-S; Protein Sa; Vitamin K Dependent Protein S

ACTIVITY TEST

Protein S (PROS) is a vitamin K-dependent plasma protein functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation.It acts as a cofactor for activated protein C, which inactivates factors Va and VIIIa, thus reducing the risk of thrombosis.Coagulation factor VIII (F8) binds to protein S (PROS) to participate in the clotting process, regulating thrombin production and the conversion of fibrinogen to fibrin, thereby maintaining normal blood clotting function.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human PROS and recombinant dog F8.Briefly, PROS was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to F8-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PROS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human PROS and recombinant dog F8 was shown in Figure 1, the EC50 for this effect is 0.058ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Arthritis Res Ther Validation in the ESPOIR cohort of vitamin K-dependent protein S (PROS) as a potential biomarker capable of predicting response to the methotrexate … Pubmed:35313956
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB971Hu02 Active Protein S (PROS) Cell culture; Activity Assays.
RPB971Hu01 Recombinant Protein S (PROS) Positive Control; Immunogen; SDS-PAGE; WB.
RPB971Hu02 Recombinant Protein S (PROS) Positive Control; Immunogen; SDS-PAGE; WB.
PAB971Hu01 Polyclonal Antibody to Protein S (PROS) WB; IHC; ICC; IP.
PAB971Hu02 Polyclonal Antibody to Protein S (PROS) WB; IHC; ICC; IP.
LAB971Hu71 Biotin-Linked Polyclonal Antibody to Protein S (PROS) WB; IHC; ICC.
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LAB971Hu72 Biotin-Linked Monoclonal Antibody to Protein S (PROS) WB; IHC; ICC.
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LMB971Hu Multiplex Assay Kit for Protein S (PROS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.