Active Annexin A2 (ANXA2)
ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV
- Product No.APB944Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point7.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 274
For more details, please contact local distributors! US$ 684 US$ 1368 US$ 4104 US$ 10260
ACTIVITY TEST
Annexin A2 (ANXA2) also known as annexin II is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. ANXA2 is up-regulated in various tumor types and plays multiple roles in regulating cellular functions, including angiogenesis, proliferation, apoptosis, cell migration, invasion and adhesion. To test the effect of ANXA2 on cell proliferation, Hela cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM prior to the addition of various concentrations of recombinant human ANXA2. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of Hela cells after incubation with ANXA2 for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant ANXA2 for 96h. The result was shown in Figure 2. It was obvious that ANXA2 significantly increased cell viability of Hela cells. (A) Hela cells cultured in DMEM, stimulated with 10ng/mL ANXA2 for 96h;
(B) Unstimulated Hela cells cultured in DMEM for 96h.<br/>Figure. Cell proliferation of Hela cells after stimulated with ANXA2.
Figure. Cell proliferation of Hela cells after stimulated with ANXA2.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Buffer
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
Magazine | Citations |
Journal of Cellular Biochemistry | Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis PubMed: 20225235 |
World Journal of Gastroenterology | Expression characteristics and diagnostic value of annexin A2 in hepatocellular carcinoma PubMed: PMC3491596 |
Oncology Letters | Annexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosis PubMed: PMC3742823 |
Ibrahim | Annexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular Carcinoma Ghrnet: Source |
European Journal of Clinical Microbiology & Infectious Diseases | Serum annexin A2 levels in acute brucellosis and brucellar spondylodiscitis Pubmed:24853056 |
Biomedicine & Pharmacotherapy | Circulating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variables Pubmed:25661364 |
Hepat Mon | Serum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis B PubMed: 26587036 |
Tumour Biol | Up-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinoma PubMed: 26109000 |
Biomed Pharmacother | Circulating PubMed: 25661364 |
Tumour Biol | Evaluation of annexin A2 and as potential biomarkers for hepatocellular carcinoma PubMed: 26189841 |
Eur J Clin Invest | Stairs instead of elevators at the workplace decreases PCSK9 levels in a healthy population PubMed: 26081791 |
Journal of Oral and Maxillofacial Surgery | Serum Annexin A2 Level Is Associated With Diagnosis and Prognosis in Patients With OralSquamous Cell Carcinoma. pubmed:27889534 |
Pregnancy Hypertens | Assessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study. pubmed:28501283 |
Mol Nutr Food Res. | Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells. pubmed:28181408 |
Archives of Gynecology and Obstetrics | Expression of annexin A2 in adenomyosis and dysmenorrhea Pubmed: 31183557 |
Cancers | Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer Pubmed: 31842290 |
PLASMA BIOMARKER FOR OVARIAN CANCER | |
Diagnostics | Diagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 69 33406648 |
ADVANCED RESEARCHES | Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C |
Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins |
Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPB944Hu03 | Recombinant Annexin A2 (ANXA2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB944Hu01 | Recombinant Annexin A2 (ANXA2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB944Hu04 | Recombinant Annexin A2 (ANXA2) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB944Hu01 | Active Annexin A2 (ANXA2) | Cell culture; Activity Assays. |
RPB944Hu02 | Recombinant Annexin A2 (ANXA2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB944Hu01 | Polyclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC; IP. |
PAB944Hu02 | Polyclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC; IP; FCM |
LAB944Hu71 | Biotin-Linked Polyclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC. |
MAB944Hu22 | Monoclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC; IP. |
MAB944Hu21 | Monoclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC; IP. |
FAB944Hu08 | Anti-Annexin A2 (ANXA2) Monoclonal Antibody | Flow cytometry. |
FAB944Hu06 | Anti-Annexin A2 (ANXA2) Monoclonal Antibody | Flow cytometry. |
FAB944Hu04 | Anti-Annexin A2 (ANXA2) Monoclonal Antibody | Flow cytometry. |
FAB944Hu02 | Anti-Annexin A2 (ANXA2) Monoclonal Antibody | Flow cytometry. |
SEB944Hu | ELISA Kit for Annexin A2 (ANXA2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB944Hu | CLIA Kit for Annexin A2 (ANXA2) | Chemiluminescent immunoassay for Antigen Detection. |
LMB944Hu | Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |