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Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay)
ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB944Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 415
US$ 431
US$ 455
US$ 487
US$ 519
US$ 567
US$ 638
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 80-95 | 88 |
EDTA plasma(n=5) | 85-99 | 94 |
heparin plasma(n=5) | 84-101 | 93 |
sodium citrate plasma(n=5) | 94-101 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 91-102% | 98-105% | 80-90% | 80-101% |
EDTA plasma(n=5) | 80-101% | 78-96% | 97-104% | 79-96% |
heparin plasma(n=5) | 83-99% | 86-95% | 82-92% | 96-104% |
sodium citrate plasma(n=5) | 80-92% | 89-103% | 93-105% | 90-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:ANXA2) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Journal of Cellular Biochemistry | Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis PubMed: 20225235 |
World Journal of Gastroenterology | Expression characteristics and diagnostic value of annexin A2 in hepatocellular carcinoma PubMed: PMC3491596 |
Oncology Letters | Annexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosis PubMed: PMC3742823 |
Ibrahim | Annexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular Carcinoma Ghrnet: Source |
European Journal of Clinical Microbiology & Infectious Diseases | Serum annexin A2 levels in acute brucellosis and brucellar spondylodiscitis Pubmed:24853056 |
Biomedicine & Pharmacotherapy | Circulating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variables Pubmed:25661364 |
Hepat Mon | Serum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis B PubMed: 26587036 |
Tumour Biol | Up-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinoma PubMed: 26109000 |
Biomed Pharmacother | Circulating PubMed: 25661364 |
Tumour Biol | Evaluation of annexin A2 and as potential biomarkers for hepatocellular carcinoma PubMed: 26189841 |
Eur J Clin Invest | Stairs instead of elevators at the workplace decreases PCSK9 levels in a healthy population PubMed: 26081791 |
Journal of Oral and Maxillofacial Surgery | Serum Annexin A2 Level Is Associated With Diagnosis and Prognosis in Patients With OralSquamous Cell Carcinoma. pubmed:27889534 |
Pregnancy Hypertens | Assessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study. pubmed:28501283 |
Mol Nutr Food Res. | Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells. pubmed:28181408 |
Archives of Gynecology and Obstetrics | Expression of annexin A2 in adenomyosis and dysmenorrhea Pubmed: 31183557 |
Cancers | Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer Pubmed: 31842290 |
PLASMA BIOMARKER FOR OVARIAN CANCER | |
Diagnostics | Diagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 69 33406648 |
ADVANCED RESEARCHES | Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C |
Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins |
Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPB944Hu03 | Recombinant Annexin A2 (ANXA2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB944Hu01 | Recombinant Annexin A2 (ANXA2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB944Hu04 | Recombinant Annexin A2 (ANXA2) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB944Hu01 | Active Annexin A2 (ANXA2) | Cell culture; Activity Assays. |
RPB944Hu02 | Recombinant Annexin A2 (ANXA2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB944Hu01 | Polyclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC; IP. |
PAB944Hu02 | Polyclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC; IP; FCM |
LAB944Hu71 | Biotin-Linked Polyclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC. |
MAB944Hu22 | Monoclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC; IP. |
MAB944Hu21 | Monoclonal Antibody to Annexin A2 (ANXA2) | WB; IHC; ICC; IP. |
FAB944Hu08 | Anti-Annexin A2 (ANXA2) Monoclonal Antibody | Flow cytometry. |
FAB944Hu06 | Anti-Annexin A2 (ANXA2) Monoclonal Antibody | Flow cytometry. |
FAB944Hu04 | Anti-Annexin A2 (ANXA2) Monoclonal Antibody | Flow cytometry. |
FAB944Hu02 | Anti-Annexin A2 (ANXA2) Monoclonal Antibody | Flow cytometry. |
SEB944Hu | ELISA Kit for Annexin A2 (ANXA2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB944Hu | CLIA Kit for Annexin A2 (ANXA2) | Chemiluminescent immunoassay for Antigen Detection. |
LMB944Hu | Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |