≡

Active Syndecan 4 (SDC4)

SYND4; Amphiglycan; Ryudocan; Ryudocan core protein

ACTIVITY TEST

Syndecan-4 (SDC4), a transmembrane proteoglycan bearing heparan sulfate chains, is an important member of SDCs family.SDC4 is expressed on dendritic cells (DCs) and activated T cells, plays a crucial role in DC motility and has been shown as a potential target for activated T-cell-driven diseases.SDC4 is involved in numerous inside-out and outside-in signaling processes, such as binding and sequestration of growth factors and extracellular matrix components, regulation of the activity of the small GTPase Rac1, protein kinase C-alpha, the level of intracellular calcium, or the phosphorylation of focal adhesion kinase. A functional binding ELISA assay was conducted to detect the interaction of recombinant human Fibroblast Growth Factor 2, Basic (FGF2) and recombinant human SDC4. Briefly, biotin-linked SDC4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGF2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human SDC4 and recombinant human FGF2 was shown in Figure 1, the EC50 for this effect is 1.45 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Lipids in Health and Disease The influence of atorvastatin on parameters of inflammation left ventricular function, hospitalizations and mortality in patients with dilated cardiomyopathy–5-year follow-up PubMed: PMC3641983
The FASEB Journal Matrix metalloproteinase 9-mediated shedding of syndecan 4 in response to tumor necrosis factor α: a contributor to endothelial cell glycocalyx dysfunction Pubmed:25122554
Scientific Reports Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153
Int J Mol Sci The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599
Biochemical and Biophysical Research Communications Syndecan-4 shedding impairs macrovascular angiogenesis in diabetes mellitus Pubmed:27018253
Kidney International Aldosterone induces albuminuria via matrix metalloproteinase–dependent damage of the endothelial glycocalyx Pubmed: 30389198
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB939Hu01 Active Syndecan 4 (SDC4) Cell culture; Activity Assays.
RPB939Hu01 Recombinant Syndecan 4 (SDC4) Positive Control; Immunogen; SDS-PAGE; WB.
PAB939Hu01 Polyclonal Antibody to Syndecan 4 (SDC4) WB; IHC; ICC; IP.
MAB939Hu22 Monoclonal Antibody to Syndecan 4 (SDC4) WB; IHC; ICC; IP.
SEB939Hu ELISA Kit for Syndecan 4 (SDC4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB939Hu Multiplex Assay Kit for Syndecan 4 (SDC4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.