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Active Apolipoprotein C3 (APOC3)

Apo-C3; APOCIII; APOC-III

ACTIVITY TEST

Apolipoprotein C-III also known as apo-CIII, and apolipoprotein C3, is a glycoprotein (consisting of 79 amino acids) that is synthesized principally in the liver. APOC3 is a surface component of chylomicrons, very low density lipoproteins, and high densityl ipoprotein and it has been recently recognized as a polyhedric factor which may regulate several pathways beyond lipid metabolism by influencing cardiovascular, metabolic, and neurological disease risk. Besides, Apolipoprotein H (APOH) has been identified as an interactor of APOC3, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human APOC3 and recombinant human APOH. Briefly, biotin-linked APOC3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APOH-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human APOC3 and recombinant human APOH was shown in Figure 1, the EC50 for this effect is 8.5 ug/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Atherosclerosis Up-reCavia (Guinea pig )lation of Hnf1α gene expression in the liver of rats with experimentally induced chronic renal failure – A possible link between circulating PCSK9 and triacylglycerol concentrations Pubmed:26978583
The Journal of Lipid Research Effects of angiopoietin-like protein 3 deficiency on postprandial lipid and lipoprotein metabolism Pubmed:27040449
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans 33407555
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB890Hu01 Active Apolipoprotein C3 (APOC3) Cell culture; Activity Assays.
RPB890Hu03 Recombinant Apolipoprotein C3 (APOC3) Positive Control; Immunogen; SDS-PAGE; WB.
RPB890Hu02 Recombinant Apolipoprotein C3 (APOC3) Positive Control; Immunogen; SDS-PAGE; WB.
RPB890Hu01 Recombinant Apolipoprotein C3 (APOC3) Positive Control; Immunogen; SDS-PAGE; WB.
PAB890Hu01 Polyclonal Antibody to Apolipoprotein C3 (APOC3) WB; ICC; IHC-P; IHC-F; ELISA.
MAB890Hu22 Monoclonal Antibody to Apolipoprotein C3 (APOC3) WB; IHC; ICC; IP.
SEB890Hu ELISA Kit for Apolipoprotein C3 (APOC3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB890Hu Multiplex Assay Kit for Apolipoprotein C3 (APOC3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.