Active Myostatin (MSTN)
GDF8; Growth Differentiation Factor 8
- Product No.APB653Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point6.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 310
For more details, please contact local distributors! US$ 774 US$ 1548 US$ 4644 US$ 11610
ACTIVITY TEST
Myostatin (MSTN) also known as growth differentiation factor 8(GDF-8) is a member of the TGF beta protein family. Myostatin is aa secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE
Reconstitute in 10mM PBS to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Buffer
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
Magazine | Citations |
Clinical Medicine and Diagnostics | Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats Sapub:Source |
Nutr Metab (Lond) | Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice PubMed: 26539241 |
International Journal of Cancer | Tumor inoculation site affects the development of cancer cachexia and muscle wasting PubMed: 26016447 |
Connect Tissue Res | Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts PubMed: 25748814 |
PLoS One | Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease Pubmed:26998756 |
Calcif Tissue Int. | The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats Pubmed:27272029 |
Archives of medical research | Irisin and Myostatin Levels in Patients with Graves' Disease. pubmed:27986127 |
Calcified Tissue International | Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats pubmed:29242963 |
Scientific Reports | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. pubmed:28740214 |
Journal of Animal Science | Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs Pubmed: 30203098 |
European Journal of Internal Medicine | Predictive value of serum myostatin for the severity and clinical outcome of heart failure Pubmed: 31056368 |
J Cell Physiol | Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats Pubmed: 31960436 |
NEPHROLOGY DIALYSIS TRANSPLANTATION | Myostatin and muscle atrophy during chronic kidney disease Pubmed: 32974666 |
Oxid Med Cell Longev | Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance … 34868459 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
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LMB653Hu | Multiplex Assay Kit for Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
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