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CLIA Kit for Myostatin (MSTN)
GDF8; Growth Differentiation Factor 8
- Product No.SCB653Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range55-40,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 26.7pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 588
For more details, please contact local distributors! US$ 840 US$ 3780 US$ 7140 US$ 58800
Specificity
This assay has high sensitivity and excellent specificity for detection of Myostatin (MSTN).
No significant cross-reactivity or interference between Myostatin (MSTN) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Myostatin (MSTN) and the recovery rates were calculated by comparing the measured value to the expected amount of Myostatin (MSTN) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 93-101 | 97 |
EDTA plasma(n=5) | 79-101 | 93 |
heparin plasma(n=5) | 87-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myostatin (MSTN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myostatin (MSTN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myostatin (MSTN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-88% | 88-95% | 91-101% | 85-105% |
EDTA plasma(n=5) | 92-99% | 88-96% | 79-97% | 97-105% |
heparin plasma(n=5) | 97-104% | 78-96% | 81-97% | 95-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
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Magazine | Citations |
Clinical Medicine and Diagnostics | Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats Sapub:Source |
Nutr Metab (Lond) | Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice PubMed: 26539241 |
International Journal of Cancer | Tumor inoculation site affects the development of cancer cachexia and muscle wasting PubMed: 26016447 |
Connect Tissue Res | Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts PubMed: 25748814 |
PLoS One | Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease Pubmed:26998756 |
Calcif Tissue Int. | The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats Pubmed:27272029 |
Archives of medical research | Irisin and Myostatin Levels in Patients with Graves' Disease. pubmed:27986127 |
Calcified Tissue International | Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats pubmed:29242963 |
Scientific Reports | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. pubmed:28740214 |
Journal of Animal Science | Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs Pubmed: 30203098 |
European Journal of Internal Medicine | Predictive value of serum myostatin for the severity and clinical outcome of heart failure Pubmed: 31056368 |
J Cell Physiol | Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats Pubmed: 31960436 |
NEPHROLOGY DIALYSIS TRANSPLANTATION | Myostatin and muscle atrophy during chronic kidney disease Pubmed: 32974666 |
Oxid Med Cell Longev | Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance … 34868459 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
APB653Hu01 | Active Myostatin (MSTN) | Cell culture; Activity Assays. |
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RPB653Hu01 | Recombinant Myostatin (MSTN) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB653Hu61 | Active Myostatin (MSTN) | Cell culture; Activity Assays. |
CPB653Hu21 | OVA Conjugated Myostatin (MSTN) | Immunogen; SDS-PAGE; WB. |
PAB653Hu08 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
PAB653Hu06 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
PAB653Hu01 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
LAB653Hu71 | Biotin-Linked Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC. |
MAB653Hu23 | Monoclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
MAB653Hu22 | Monoclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
MAB653Hu21 | Monoclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
SEB653Hu | ELISA Kit for Myostatin (MSTN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEB653Hu | ELISA Kit for Myostatin (MSTN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB653Hu | CLIA Kit for Myostatin (MSTN) | Chemiluminescent immunoassay for Antigen Detection. |
LMB653Hu | Multiplex Assay Kit for Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
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