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Active C4 Binding Protein Alpha (C4BPa)

C4BP-a; PRP; C4BP; Complement Component 4 Binding Protein,Alpha; Proline-rich protein

ACTIVITY TEST

C4BPa, also known as C4b-binding protein alpha chain or C4BPalpha, is a subunit of the complement factor C4b-binding protein (C4BP), which plays a crucial role in the regulation of the complement system, a part of the immune system that helps to defend the body against infection.C4BPa is part of a heterodimeric protein complex that consists of two chains, the alpha chain (C4BPa) and the beta chain (C4BPB). It can bind to C4b and C3b, thereby inhibiting the formation of the C3 convertase and preventing excessive complement activation. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C4BPa and recombinant mouse C4b. Briefly, C4BPa was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to C4b-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C4BPa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C4BPa and recombinant mouse C4b was shown in Figure 1, the EC50 for this effect is 0.01 ug/mL

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Vascular Surgery Circulation levels of acute phase proteins in patients with Takayasu arteritis PubMed: 20100644
British Journal of Cancer Identification of a novel serum biomarker for pancreatic cancer, C4b-binding protein α-chain (C4BPA) by quantitative proteomic analysis using tandem mass tags. pubmed:27657339
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB620Hu01 Recombinant C4 Binding Protein Alpha (C4BPa) Positive Control; Immunogen; SDS-PAGE; WB.
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