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Active Thymosin Beta 4 (TMSB4X)

Tb4; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide

ACTIVITY TEST

Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta-thymosin family of molecules. It Plays an important role in the organization of the cytoskeleton. It is reported that TMSB4X can combinant with PFN1 to inhibit actin polymerization. A binding ELISA assay was conducted to detect the interaction of recombinant human TMSB4X and recombinant human PFN1. Briefly, TMSB4X were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to PFN1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TMSB4X pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TMSB4X and PFN1 was shown in Figure 1, the EC50 for this effect is 2.98 ng/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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PAB609Hu01 Polyclonal Antibody to Thymosin Beta 4 (TMSB4X) WB; IHC; ICC; IP.
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LAB609Hu71 Biotin-Linked Polyclonal Antibody to Thymosin Beta 4 (TMSB4X) WB; IHC; ICC.
MAB609Hu21 Monoclonal Antibody to Thymosin Beta 4 (TMSB4X) WB; IHC; ICC; IP.
CEB609Hu ELISA Kit for Thymosin Beta 4 (TMSB4X) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB609Hu Multiplex Assay Kit for Thymosin Beta 4 (TMSB4X) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.