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Active Aryl Hydrocarbon Receptor (AhR)

BHLHE76; Class E basic helix-loop-helix protein 76

ACTIVITY TEST

Aryl Hydrocarbon Receptor (AhR), a protein of substantial molecular mass, typically ranging from 110 to 150 kilodaltons, is a member of the esteemed Basic Helix-loop-helix PAS (Per-Arnt-Sim) family of transcription factors. Ubiquitously expressed in vertebrate cells, AhR's activity is meticulously modulated by a variety of ligands. This receptor is pivotal in orchestrating gene transcription, thereby wielding considerable influence over a multitude of biological processes.Aryl Hydrocarbon Receptor Interacting Protein (AIP)can combine with AHR to regulate the endocrine system.Thus a functional ELISA assay was conducted to detect the interaction of recombinant recombinant mouse AhR and recombinant human AIP. Briefly, AhR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to AIP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AhR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant recombinant mouse AhR and recombinant human AIP was shown in Figure 1, the EC50 for this effect is 0.49ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Molecular and Cellular Endocrinology Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells Pubmed:24859647
Journal of Steroid Biochemistry and Molecular Biology Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910
Biol Trace Elem Res Investigation of Aryl Hydrocarbon Receptor, Zinc, and Vitamin B12 Levels in Chronic Gastritis with Helicobacter pylori Infection 33723799
Int J Clin Pract Association of aryl hydrocarbon receptor (AhR) serum level and gene rs10247158 polymorphism with anthropometric, biochemical parameters and food consumption ¡­ 34091989
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APB354Mu01 Active Aryl Hydrocarbon Receptor (AhR) Cell culture; Activity Assays.
RPB354Mu01 Recombinant Aryl Hydrocarbon Receptor (AhR) Positive Control; Immunogen; SDS-PAGE; WB.
PAB354Mu01 Polyclonal Antibody to Aryl Hydrocarbon Receptor (AhR) WB
SEB354Mu ELISA Kit for Aryl Hydrocarbon Receptor (AhR) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB354Mu Multiplex Assay Kit for Aryl Hydrocarbon Receptor (AhR) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.