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Active Aryl Hydrocarbon Receptor (AhR)

BHLHE76; Class E basic helix-loop-helix protein 76

ACTIVITY TEST

Aryl Hydrocarbon Receptor (AhR), a protein of substantial molecular mass, typically ranging from 110 to 150 kilodaltons, is a member of the esteemed Basic Helix-loop-helix PAS (Per-Arnt-Sim) family of transcription factors. Ubiquitously expressed in vertebrate cells, AhR's activity is meticulously modulated by a variety of ligands. This receptor is pivotal in orchestrating gene transcription, thereby wielding considerable influence over a multitude of biological processes.Aryl Hydrocarbon Receptor Interacting Protein (AIP)can combine with AHR to regulate the endocrine system.Thus a functional ELISA assay was conducted to detect the interaction of recombinant recombinant mouse AhR and recombinant human AIP. Briefly, AhR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to AIP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AhR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant recombinant mouse AhR and recombinant human AIP was shown in Figure 1, the EC50 for this effect is 0.49ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Molecular and Cellular Endocrinology Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells Pubmed:24859647
Journal of Steroid Biochemistry and Molecular Biology Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910
Biol Trace Elem Res Investigation of Aryl Hydrocarbon Receptor, Zinc, and Vitamin B12 Levels in Chronic Gastritis with Helicobacter pylori Infection 33723799
Int J Clin Pract Association of aryl hydrocarbon receptor (AhR) serum level and gene rs10247158 polymorphism with anthropometric, biochemical parameters and food consumption ¡­ 34091989
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APB354Mu01 Active Aryl Hydrocarbon Receptor (AhR) Cell culture; Activity Assays.
RPB354Mu01 Recombinant Aryl Hydrocarbon Receptor (AhR) Positive Control; Immunogen; SDS-PAGE; WB.
PAB354Mu01 Polyclonal Antibody to Aryl Hydrocarbon Receptor (AhR) WB
SEB354Mu ELISA Kit for Aryl Hydrocarbon Receptor (AhR) Enzyme-linked immunosorbent assay for Antigen Detection.
CMB354Mu Multiplex Assay Kit for Aryl Hydrocarbon Receptor (AhR) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.
LMB354Mu Multiplex Assay Kit for Aryl Hydrocarbon Receptor (AhR) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.