≡

Active Cathepsin D (CTSD)

CPSD; CLN10; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis,Neuronal 10

ACTIVITY TEST

Cathepsin D is a lysosomal aspartic protease of the pepsin family. Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 118), a propeptide (residues 1964), and a mature chain (residues 65412). The mature chain can be processed further to the light (residues 65161) and heavy (residues 169412) chains. It is expressed in most cells and overexpressed in breast cancer cells. It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis. The activity of recombinant human CTSD is measured by its ability to cleave a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 0.1 M NaOAc, 0.2 M NaCl, pH 3.5. The rhCTSD is diluted to 20 ug/ml in assay buffer, then incubated at 37 °C for 30min. The incubated rhCTSD is diluted to 5 ug/mL in assay buffer. Loading into a black well plate 50 µL of 5 ug/mL rhCTSD and start the reaction by adding 50 µL of 60 µM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rhCTSD. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human CTSD is > 675 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Immunology Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297
21 Plasma Cathepsin D Levels: A Novel Tool to Predict Pediatric Hepatic Inflammation PubMed: 25732418
Scientific Reportes Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention pubmed:27922112
Biomedicine & pharmacotherapy Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms pubmed:28061403
JGH OPEN Limited applicability of cathepsin D for the diagnosis and monitoring of non‐alcoholic steatohepatitis
Diabetologia Plasma cathepsin D activity is negatively associated with hepatic insulin sensitivity in overweight and obese humans Pubmed: 31690989
Antioxidants Iron and Advanced Glycation End Products: Emerging Role of Iron in Androgen Deficiency in Obesity Pubmed: 32235809
Peptides LVV-hemorphin-7 (LVV-H7) plays a role in antinociception in a rat model of alcohol-induced pain disorders 33253777
Cells Identification of Cathepsin D as a Plasma Biomarker for Alzheimer's Disease 33445607
J Chem Neuroanat Analysis of silymarin-modulating effects against acrylamide-induced cerebellar damage in male rats: Biochemical and pathological markers 33965515
Front Cell Infect Microbiol PGRS Domain of Rv0297 of Mycobacterium tuberculosis Is Involved in Modulation of Macrophage Functions to Favor Bacterial Persistence 33042856
Nutr Metab Cardiovasc Dis Serum CathepsinD in pregnancy: relation with metabolic and inflammatory markers and effects of fish oils and probiotics Pubmed:35304048
Biosensors A Comparison of Various Chips Used for the Manufacture of Biosensors Applied in Non-Fluidic Array SPRi, Based on the Example of Determination of Cathepsin D Pubmed:35049649
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB280Hu61 Active Cathepsin D (CTSD) Cell culture; Activity Assays.
EPB280Hu61 Eukaryotic Cathepsin D (CTSD) Positive Control; Immunogen; SDS-PAGE; WB.
RPB280Hu01 Recombinant Cathepsin D (CTSD) Positive Control; Immunogen; SDS-PAGE; WB.
APB280Hu62 Active Cathepsin D (CTSD) Cell culture; Activity Assays.
EPB280Hu62 Eukaryotic Cathepsin D (CTSD) Positive Control; Immunogen; SDS-PAGE; WB.
RPB280Hu02 Recombinant Cathepsin D (CTSD) Positive Control; Immunogen; SDS-PAGE; WB.
RPB280Hu03 Recombinant Cathepsin D (CTSD) Positive Control; Immunogen; SDS-PAGE; WB.
RPB280Hu04 Recombinant Cathepsin D (CTSD) Positive Control; Immunogen; SDS-PAGE; WB.
PAB280Hu02 Polyclonal Antibody to Cathepsin D (CTSD) WB; IHC; ICC; IP.
PAB280Hu01 Polyclonal Antibody to Cathepsin D (CTSD) WB; IHC; ICC; IP.
LAB280Hu71 Biotin-Linked Polyclonal Antibody to Cathepsin D (CTSD) WB; IHC; ICC.
MAB280Hu21 Monoclonal Antibody to Cathepsin D (CTSD) WB; IHC; ICC; IP.
MAB280Hu26 Monoclonal Antibody to Cathepsin D (CTSD) WB; IHC; ICC; IP.
MAB280Hu22 Monoclonal Antibody to Cathepsin D (CTSD) WB; IHC; ICC; IP.
FAB280Hu02 Anti-Cathepsin D (CTSD) Monoclonal Antibody Flow cytometry.
SEB280Hu ELISA Kit for Cathepsin D (CTSD) Enzyme-linked immunosorbent assay for Antigen Detection.
HEB280Hu High Sensitive ELISA Kit for Cathepsin D (CTSD) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB280Hu Multiplex Assay Kit for Cathepsin D (CTSD) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.