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Active Glutathione S Transferase Pi (GSTp)

GSTP1; GST-P1; DFN7; FAEES3; GST3

ACTIVITY TEST

Glutathione S-transferase P (GSTP1) is a member of Glutathione S-transferases (GSTs) family which play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340nm. The reaction was performed in adding 10μL 200mM glutathione (reduced) and 10μL 100mM CDNB in 980μL 100mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50μL mixed substrates to 50μL recombinant rat GSTP1, mix gentliy, read the absorbance at 340nm for 5min. One unit activity was defined as 1 μmole of CDNB conjugate per minute per 1 mg GSTP1

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
International Journal of Medical Sciences Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma PubMed: PMC3619117
Mol Neurobiol Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis Pubmed:27021019
Journal of Biomaterials and Tissue Engineering 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering art00007
Neuroscience Bulletin Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state Pubmed:30019218
Arabian Journal of Chemistry Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats
International Journal of Nature and Life Sciences The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands
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