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Active Glutathione S Transferase Pi (GSTp)

GSTP1; GST-P1; DFN7; FAEES3; GST3

ACTIVITY TEST

Recombinant Glutathione S Transferase Pi (GSTp) is a key enzyme in the body. It conjugates glutathione with electrophilic substances, playing a vital role in detoxification and antioxidant defense. GSTp is involved in protecting cells from harmful substances and oxidative stress by binding with PRDX6 .Thus a functional ELISA assay was conducted to detect the interaction of recombinant rat GSTp and recombinant rat PRDX6. Briefly, GSTp was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PRDX6-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat GSTp and recombinant rat PRDX6 was shown in Figure 1, the EC50 for this effect is 0.008ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
International Journal of Medical Sciences Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma PubMed: PMC3619117
Mol Neurobiol Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis Pubmed:27021019
Journal of Biomaterials and Tissue Engineering 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering art00007
Neuroscience Bulletin Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state Pubmed:30019218
Arabian Journal of Chemistry Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats
International Journal of Nature and Life Sciences The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands
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