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Active Gamma-synuclein (SNCG)

SNC-G; gSYN; SR; BCSG1; PERSYN; PRSN; Breast Cancer Specific Protein 1; Persyn; Synoretin; Synuclein Gamma

ACTIVITY TEST

Gamma-synuclein (SNCG), a 127-amino-acid protein belonging to the synuclein family, is predominantly expressed in the nervous system and epithelial tissues. It regulates cytoskeletal dynamics, cell proliferation, and neurotransmitter release under physiological conditions. Dysregulated SNCG expression is linked to neurodegenerative diseases and multiple cancers, where it promotes tumor invasion and metastasis by modulating signaling pathways. Emerging evidence indicates its role as an oncogenic driver, making it a potential therapeutic target. SNCG interacts with VEGFR1 to enhance vascular endothelial growth factor (VEGF)-mediated signaling, facilitating angiogenesis and tumor progression.To detect the activity of recombinant SNCG, a functional ELISA assay was performed to evaluate the interaction between recombinant human SNCG and recombinant human VEGFR1.Briefly, biotin-linked SNCG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to VEGFR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of SNCG and VEGFR1 was shown in Figure 1, the EC50 for this effect is 0.15147µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APA939Mu01 Active Gamma-synuclein (SNCG) Cell culture; Activity Assays.
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