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Active Resistin (RETN)
ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3
- Product No.APA847Hu62
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.8
- ApplicationsCell culture; Activity Assays.
- Downloadn/a
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
ACTIVITY TEST
Resistin (RETN), named for its association with insulin resistance, is a cysteine-rich adipokine primarily secreted by immune cells like macrophages in humans, as well as by adipocytes in rodents. It plays a key role in promoting insulin resistance, inflammation, and endothelial dysfunction. Resistin is implicated in the pathogenesis of type 2 diabetes, obesity, and atherosclerosis by interfering with insulin signaling pathways and increasing the production of pro-inflammatory cytokines. Its circulating levels are often elevated in metabolic disorders, making it a potential biomarker and therapeutic target for these conditions. The mechanisms of action involve interactions with various receptors, such as Toll-like Receptor 4 (TLR4) and Adenylyl Cyclase-Associated Protein 1 (CAP1), leading to the activation of inflammatory signaling cascades.Besides,CFD has been identified as an interactor of RETN, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human RETN and recombinant human CFD . Briefly, biotin-linked RETN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to CFD-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of RETN and CFD was shown in Figure 1, the EC50 for this effect is 2.04126µg/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| European Journal of Pharmacology | Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961 |
| Evid Based Complement Alternat Med. | Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645 |
| J Endocrinol. | Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle Pubmed:24681828 |
| Life Sciences | Adipose tissue regulates hepatic cholesterol metabolism via adiponectin Pubmed:25445438 |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126 |
| Evid Based Complement Alternat Med | Tinospora crispa PubMed: 25821506 |
| Journal of | Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysis PubMed: 26686707 |
| Genome | Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without Complications PubMed: 25120108 |
| Acta Histochemica | Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234 |
| Journal of Dairy Science | Endocrine activity of adipose tissues as influenced by energy intake in the periparturient cow 108495429X:34 |
| Evidence-Based Complementary and Alternative Medicine | Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535 |
| Oncotarget | FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway pubmed:28515350 |
| Journal of Dairy Science | Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levels pubmed:28803009 |
| Toxicology | Biological effects of adipocytes in sulfur mustard induced toxicity pubmed:29129815 |
| Breast Cancer Research | Bashar Zeidan1, Antigoni Manousopoulou2, Diana J. Garay-Baquero2, 3, Cory H. White3, 7, Samantha ET Larkin1, Kathleen N. Potter1, Theodoros I … Pubmed:29566726 |
| Bioscience Reports | Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats Pubmed:29784871 |
| Oncotarget | Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120 |
| Journal of Functional Foods | Mango leaf tea promotes hepatoprotective effects in obese rats Doi: 10.1016/j.jff.2018.09.010 |
| Food Research International | Bacupari (Garcinia brasiliensis) extract modulates intestinal microbiota and reduces oxidative stress and inflammation in obese rats Doi: 10.1016/j.foodres.2019.04.012 |
| Journal of Functional Foods | Extruded sorghum (Sorghum bicolor L.) improves gut microbiota, reduces inflammation, and oxidative stress in obese rats fed a high-fat diet |
| Nat Commun | Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncoupler Pubmed: 31092829 |
| Nutrients | Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of … Pubmed: 31514318 |
| GYNECOLOGICAL ENDOCRINOLOGY | Saliva resistin as a screening marker of gestational diabetes mellitus Pubmed: 32804001 |
| Chinese Journal of Natural Medicines | Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles Pubmed: 32768169 |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body … Pubmed: 32545900 |
| Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin … 34438908 | |
| High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients | |
| Skeletal Muscle Fatty Acid Metabolism is Diverted Towards the Synthesis of Signaling Intermediates Without Fatty Acid Uptake Contribution in Dry Cows with Obesity … |