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Active Resistin (RETN)

ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3

ACTIVITY TEST

Resistin (resistance-to-insulin, RETN) is a 10 kDa member of a small family of secreted cysteine-rich peptide hormones which also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3). Human Resistin precursor is 108 amino acids (aa) in length. It contains an 18 aa signal sequence plus a 90 aa mature region. These molecules purportedly play some role in inflammation, glucose metabolism, and angiogenesis. Besides, Toll Like Receptor 4 (TLR4) has been identified as an interactor of RETN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RETN and recombinant human TLR4. Briefly, RETN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TLR4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-RETN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of RETN and TLR4 was shown in Figure 1, the ED50 for this effect is 5.13 ug/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
European Journal of Pharmacology Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961
Evid Based Complement Alternat Med. Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645
J Endocrinol. Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle Pubmed:24681828
Life Sciences Adipose tissue regulates hepatic cholesterol metabolism via adiponectin Pubmed:25445438
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126
Evid Based Complement Alternat Med Tinospora crispa PubMed: 25821506
Journal of Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysis PubMed: 26686707
Genome Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without Complications PubMed: 25120108
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234
Journal of Dairy Science Endocrine activity of adipose tissues as influenced by energy intake in the periparturient cow 108495429X:34
Evidence-Based Complementary and Alternative Medicine Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535
Oncotarget FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway pubmed:28515350
Journal of Dairy Science Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levels pubmed:28803009
Toxicology Biological effects of adipocytes in sulfur mustard induced toxicity pubmed:29129815
Breast Cancer Research Bashar Zeidan1, Antigoni Manousopoulou2, Diana J. Garay-Baquero2, 3, Cory H. White3, 7, Samantha ET Larkin1, Kathleen N. Potter1, Theodoros I … Pubmed:29566726
Bioscience Reports Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats Pubmed:29784871
Oncotarget Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120
Journal of Functional Foods Mango leaf tea promotes hepatoprotective effects in obese rats Doi: 10.1016/j.jff.2018.09.010
Food Research International Bacupari (Garcinia brasiliensis) extract modulates intestinal microbiota and reduces oxidative stress and inflammation in obese rats Doi: 10.1016/j.foodres.2019.04.012
Journal of Functional Foods Extruded sorghum (Sorghum bicolor L.) improves gut microbiota, reduces inflammation, and oxidative stress in obese rats fed a high-fat diet
Nat Commun Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncoupler Pubmed: 31092829
Nutrients Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of … Pubmed: 31514318
GYNECOLOGICAL ENDOCRINOLOGY Saliva resistin as a screening marker of gestational diabetes mellitus Pubmed: 32804001
Chinese Journal of Natural Medicines Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles Pubmed: 32768169
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body … Pubmed: 32545900
Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin … 34438908
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients
Skeletal Muscle Fatty Acid Metabolism is Diverted Towards the Synthesis of Signaling Intermediates Without Fatty Acid Uptake Contribution in Dry Cows with Obesity …
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPA847Hu61 Eukaryotic Resistin (RETN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA847Hu01 Recombinant Resistin (RETN) Positive Control; Immunogen; SDS-PAGE; WB.
APA847Hu61 Active Resistin (RETN) Cell culture; Activity Assays.
PAA847Hu06 Polyclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
PAA847Hu01 Polyclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
LAA847Hu81 FITC-Linked Polyclonal Antibody to Resistin (RETN) WB; IHC; ICC; IF.
LAA847Hu71 Biotin-Linked Polyclonal Antibody to Resistin (RETN) WB; IHC; ICC.
MAA847Hu27 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Hu29 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Hu23 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Hu21 Monoclonal Antibody to Resistin (RETN) IHC
MAA847Hu24 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Hu25 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Hu22 Monoclonal Antibody to Resistin (RETN) WB,IHC
LAA847Hu82 FITC-Linked Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IF.
LAA847Hu72 Biotin-Linked Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC.
SEA847Hu ELISA Kit for Resistin (RETN) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA847Hu Mini Samples ELISA Kit for Resistin (RETN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA847Hu Multiplex Assay Kit for Resistin (RETN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA847Hu01 ELISA Kit DIY Materials for Resistin (RETN) Main materials for "Do It (ELISA Kit) Yourself".