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Active Coagulation Factor IX (F9)

HEMB; FIX; GLA Domain; PTC; Anti Hemophilic Factor B; Christmas Factor; Plasma Thromboplastic Component; Christmas Disease; Hemophilia B

ACTIVITY TEST

Coagulation Factor IX, also known as Christmas Factor, is a secreted by the liver and plays a key role in the activation of the intrinsic clotting cascade. Factor IX consists of a Gla domain, two tandem EGF-like domains, an activation peptide, and an S1 serine protease domain. Mature human Factor IX shares approximately 81% amino acid sequence identity with mouse and rat Factor IX. Alternative splicing generates an additional isoform that lacks the first EGF-like domain. The activity of recombinant rabbit Coagulation Factor IX is measured by its ability to cleave a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 100 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 8.0. The Coagulation Factor IX is diluted to 100 ug/ml in assay buffer, then activated with a final concentration of 10 ug/ml Thermolysin at 37 ℃ for 90min. Adding a final concentration of 10 mM 1,10 Phenanthroline to stop the activation. The activated Coagulation Factor IX is diluted to 6 ug/mL in assay buffer. Loading into a black well plate 50 µL of 6 ug/mL Coagulation Factor IX and start the reaction by adding 50 µL of 20 uM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no Coagulation Factor IX. Then read at 320/405 nm in kinetic mode for 5 minutes. The specific activity of recombinant rabbit Coagulation Factor IX is > 20 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Cardiovascular Research Loss of accelerates arterial thrombosis by increasing formation of neutrophil extracellular traps and plasma tissue factor activity Pubmed:29444200
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
APA840Rb03 Active Coagulation Factor IX (F9) Cell culture; Activity Assays.
RPA840Rb02 Recombinant Coagulation Factor IX (F9) Positive Control; Immunogen; SDS-PAGE; WB.
RPA840Rb03 Recombinant Coagulation Factor IX (F9) Positive Control; Immunogen; SDS-PAGE; WB.
RPA840Rb01 Recombinant Coagulation Factor IX (F9) Positive Control; Immunogen; SDS-PAGE; WB.
PAA840Rb51 Polyclonal Antibody to Coagulation Factor IX (F9) WB; IHC; ICC; IP.
LAA840Rb71 Biotin-Linked Polyclonal Antibody to Coagulation Factor IX (F9) WB; IHC; ICC.
MAA840Rb21 Monoclonal Antibody to Coagulation Factor IX (F9) WB
MAA840Rb22 Monoclonal Antibody to Coagulation Factor IX (F9) WB; IHC; ICC; IP.