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Active Macrophage Migration Inhibitory Factor (MIF)
GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase
- Product No.APA698Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point6.6
- ApplicationsCell culture; Activity Assays.
- Downloadn/a
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 200
US$ 500
US$ 1000
US$ 3000
US$ 7500
For more details, please contact local distributors!
ACTIVITY TEST
Macrophage Migration Inhibitory Factor (MIF) is a pleiotropic cytokine that participates in both innate and adaptive immune responses. It is constitutively expressed in various cell types and rapidly released upon exposure to inflammatory stimuli, playing a pivotal role in regulating macrophage functions—including phagocytosis, cytokine production, and immune activation. Beyond immune regulation, MIF is also involved in modulating cell proliferation, apoptosis, and autophagy. Clinically, it has been implicated in the pathogenesis of multiple diseases, such as sepsis, autoimmune disorders, and various malignancies. Mechanistically, MIF binds to Cluster Of Differentiation 74 (CD74)—the invariant chain of major histocompatibility complex (MHC) class II complexes—with high affinity. As MIF’s primary high-affinity receptor, CD74 mediates the initiation of pro-inflammatory signaling pathways downstream of MIF binding.To detect the activity of recombinant MIF, a functional ELISA assay was performed to evaluate the interaction between recombinant human MIF and recombinant rat CD74.Briefly, biotin-linked MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to CD74-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of MIF and CD74 was shown in Figure 1, the EC50 for this effect is 0.16 µg/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Kidney International | Targeted reduction of advanced glycation improves renal function in obesity PubMed: 21412218 |
| The Journal of Immunology | Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut Jimmunol: 5673 |
| The Journal of Endocrinology | Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease PubMed: PMC3757527 |
| The Journal of Immunology | Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice PubMed: PMC3466372 |
| Diabetologia | Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes Pubmed:24957662 |
| BioMed Research International | The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder PubMed: 25705692 |
| Diabetologia | Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease. pubmed:28160092 |
| Life Sciences | Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis pubmed:28919396 |
| Scientific Reports | Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke Pubmed:29335619 |
| Ocular Immunology and Inflammation | Immune Response and Mechanisms of IFN-γ in Administration for Keratomycosis Pubmed: 30307777 |
| International Journal of Immunopathology and Pharmacology | Effect of voluntary running on expression of myokines in brains of rats with depression Pubmed: 30834799 |
| Biomarkers in Heart Failure and Associated Diseases | Biomarkers in Heart Failure and Associated Diseases |
| Science China-Life Sciences | Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation Pubmed: 31209799 |
| Folia Morphologica | Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression Pubmed: 31802473 |
| Biomed Pharmacother | Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis Pubmed: 32062385 |
| Sci Rep | Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population 33850223 |
| Commun Biol | Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes Pubmed:35115654 |