Active Slit Homolog 2 (Slit2)
SLIL3
- Product No.APA672Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point6.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 326
For more details, please contact local distributors! US$ 814 US$ 1628 US$ 4884 US$ 12210
ACTIVITY TEST
![](/upload/product/pictures/APA672Ra01-201802271708136112.jpg)
Figure. The binding activity of Slit2 with GREM1.
Slit is a family of secreted extracellular matrix proteins which play an important signalling role in the neural development of most bilaterians. Humans, mice and other vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2 protein has recently been discovered to be associated with the development of new blood vessels from pre-existing vessels, or angiogenesis. Slit2 has been implicated in promoting angiogenesis in mice (both in vitro and in vivo), in the human placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has been identified as an interactor of Slit2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GREM1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of Slit2 and GREM1 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine | Citations |
Cancer Research | SLIT2 Attenuation during Lung Cancer Progression Deregulates β-Catenin and E-Cadherin and Associates with Poor Prognosis AACR: 702543 |
Carcinogenesis | Slit-2 facilitates interaction of P-cadherin with Robo-3 and inhibits cell migration in an oral squamous cell carcinoma cell line OxfordJournals: source |
PLoS One | Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation Pubmed:26889680 |
ESC Heart Fail | Crosstalk between the activated Slit2¨CRobo1 pathway and TGF©\¦Â1 signalling promotes cardiac fibrosis 33236535 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
APA672Ra01 | Active Slit Homolog 2 (Slit2) | Cell culture; Activity Assays. |
RPA672Ra01 | Recombinant Slit Homolog 2 (Slit2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA672Ra01 | Polyclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC; IP. |
LAA672Ra71 | Biotin-Linked Polyclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC. |
MAA672Ra21 | Monoclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC; IP. |
SEA672Ra | ELISA Kit for Slit Homolog 2 (Slit2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA672Ra | Multiplex Assay Kit for Slit Homolog 2 (Slit2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |