≡

Active Slit Homolog 2 (Slit2)

SLIL3

ACTIVITY TEST

Figure. The binding activity of Slit2 with GREM1.
Slit is a family of secreted extracellular matrix proteins which play an important signalling role in the neural development of most bilaterians. Humans, mice and other vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2 protein has recently been discovered to be associated with the development of new blood vessels from pre-existing vessels, or angiogenesis. Slit2 has been implicated in promoting angiogenesis in mice (both in vitro and in vivo), in the human placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has been identified as an interactor of Slit2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GREM1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of Slit2 and GREM1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Cancer Research SLIT2 Attenuation during Lung Cancer Progression Deregulates β-Catenin and E-Cadherin and Associates with Poor Prognosis AACR: 702543
Carcinogenesis Slit-2 facilitates interaction of P-cadherin with Robo-3 and inhibits cell migration in an oral squamous cell carcinoma cell line OxfordJournals: source
PLoS One Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation Pubmed:26889680
ESC Heart Fail Crosstalk between the activated Slit2¨CRobo1 pathway and TGF©\¦Â1 signalling promotes cardiac fibrosis 33236535
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA672Ra01 Active Slit Homolog 2 (Slit2) Cell culture; Activity Assays.
RPA672Ra01 Recombinant Slit Homolog 2 (Slit2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA672Ra01 Polyclonal Antibody to Slit Homolog 2 (Slit2) WB; IHC; ICC; IP.
LAA672Ra71 Biotin-Linked Polyclonal Antibody to Slit Homolog 2 (Slit2) WB; IHC; ICC.
MAA672Ra21 Monoclonal Antibody to Slit Homolog 2 (Slit2) WB; IHC; ICC; IP.
SEA672Ra ELISA Kit for Slit Homolog 2 (Slit2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA672Ra Multiplex Assay Kit for Slit Homolog 2 (Slit2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.