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Active Nitric Oxide Synthase Trafficker (NOSTRIN)

BM247 homolog; Nitric oxide synthase traffic inducer; eNOS-trafficking inducer

ACTIVITY TEST

Nitric Oxide Synthase Trafficker (NOSTRIN) is a scaffolding protein that regulates the subcellular localization and activity of endothelial nitric oxide synthase (eNOS), a key enzyme in vascular nitric oxide (NO) production. NOSTRIN facilitates eNOS trafficking to caveolae, specialized membrane microdomains, where it modulates NO signaling. Dysregulation of NOSTRIN has been linked to vascular disorders, including preeclampsia, where its overexpression correlates with reduced eNOS activity and impaired vasodilation. NOSTRIN interacts with Caveolin 1 (CAV1), a structural protein of caveolae, through direct binding, influencing eNOS sequestration and NO bioavailability.To detect the activity of recombinant NOSTRIN, a functional ELISA assay was performed to evaluate the interaction between recombinant human NOSTRIN and recombinant human CAV1.Briefly,
NOSTRIN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CAV1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NOSTRIN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat NOSTRIN and recombinant human CAV1 was shown in Figure 1, the EC50 for this effect is 46.38 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry The pathogenetic importance of C774T single nucleotide polymorphism of the endothelial nitric oxide synthase gene in the development of metabolic syndrome article:10.1134
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA628Hu01 Active Nitric Oxide Synthase Trafficker (NOSTRIN) Cell culture; Activity Assays.
RPA628Hu01 Recombinant Nitric Oxide Synthase Trafficker (NOSTRIN) Positive Control; Immunogen; SDS-PAGE; WB.
PAA628Hu01 Polyclonal Antibody to Nitric Oxide Synthase Trafficker (NOSTRIN) IHC; ICC/IF
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LMA628Hu Multiplex Assay Kit for Nitric Oxide Synthase Trafficker (NOSTRIN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.