Active Adiponectin (ADPN)
GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
- Product No.APA605Ga01
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point6.4
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 260
For more details, please contact local distributors! US$ 650 US$ 1300 US$ 3900 US$ 9750
ACTIVITY TEST
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Human Adiponectin (ADPN) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is a plasma protein secreted by adipose tissue showing pleiotropic effects with anti-diabetic, anti-atherogenic and anti-inflammatory properties. Adiponectin and AdipoR1 regulate PPARgC1a and mitochondria by Ca(2 ) and AMPK/SIRT1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant chicken ADPN and recombinant rat PPARgC1a. Briefly, ADPN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PPARgC1a-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ADPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant chicken ADPN and recombinant rat PPARgC1a was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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BCA Protein Quantification Kit
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Molecular Mass Marker for Protein
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
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Buffer
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Lentivirus Packaging Experiment Service
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Adenovirus Packaging Experiment Service
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Magazine | Citations |
European Journal of Pharmacology | Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961 |
BioMed Research International | Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats PubMed: PMC3591142 |
PLoS One | CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and Production of Pathogenic IgG-Antibodies PubMed: PMC3297623 |
Molecular and Cellular Endocrinology | Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194 |
British Journal of Nutrition | n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577 |
International Urology Nephrology | Perirenal fat associated with microalbuminuria in obese rats Pubmed: 24526332 |
Int J Mol Sci. | Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781 |
Mol Biol Rep. | Adiponectin decreases lipids deposition by p38 MAPK/ATF2 signaling pathway in muscle of broilers. Pubmed: 24178343 |
Endocr J. | CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658 |
Iran J Basic Med Sci | Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats Pubmed:Pmc4046234 |
Biometals.? | Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats Pubmed:24519243 |
Poult Sci.? | Effect of perches on liver health of hens Pubmed:24812236 |
Gen Comp Endocrinol. | The effect of recombinant adeno-associated virus-adiponectin (rAAV2/1-Acrp30) on glycolipid dysmetabolism and liver morphology in diabetic rats Pubmed:25019654 |
Journal of Trace Elements in Medicine and Biology | Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats Pubmed:25194956 |
J Endocrinol | Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats Pubmed:25240061 |
Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126 |
Int J Clin Exp Pathol | Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway PubMed: 26617802 |
Cell Physiol Biochem | Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway PubMed: 26160485 |
Life Sciences | Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet PubMed: 26188592 |
MEDICAL RESEARCH JOURNAL | Relation between afamin level and some inflammatory markers in obese children Mrjnrc: Abstract |
Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes. Pubmed:26896925 | |
Technologia Alimentaria | Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats abstract:10 |
Obes Surg. | Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175 |
Acta Histochemica | Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234 |
Adv Biomed Res. | Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats pmc:PMC4817393 |
Biological Trace Element Research | Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats article:s12011-016-0777-1 |
Journal of Functional Foods | Antiobesity and antihyperlipidaemic effects of Yan-Sheng-Yin in animals and humans science:S1756464616300585 |
J Bone Miner Res | Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082 |
Journal of Molecular and Cellular Cardiology | Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193 |
Journal of Diabetes Research | Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose pubmed:27127795 |
Asian-Australasian Journal of Animal Sciences | Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817 |
Biomedical and Pharmacology Journal | Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case- … vol10no1 |
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES | Berberis integerrima ameliorates insulin resistance in high-fructose-fed insulin-resistant rats pubmed:29147484 |
Evidence-Based Complementary and Alternative Medicine | BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway journals:1758383 |
Oxidative Medicine and Cellular Longevity | Differential Susceptibility of Germ and Leydig Cells to Cadmium-Mediated Toxicity: Impact on Testis Structure, Adiponectin Levels, and Steroidogenesis journals:3405089 |
Iranian Journal of Pharmaceutical Research | Antihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistance pubmed:29201096 |
Biomedical & Pharmacology Journal | Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case-Control Study DOI:10.13005/bpj/1123 |
Oncotarget | FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway pubmed:28515350 |
Diabetes & metabolism | Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696 |
Frontiers in Endocrinology | Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … Pubmed:29755407 |
Food & Function | Calycosin directly improves perivascular adipose tissue dysfunction by upregulating the adiponectin/AMPK/eNOS pathway in obese mice Pubmed:29595858 |
Evidence-based Complementary and Alternative Medicine | Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling … Pubmed:29619063 |
Medicine (Baltimore) | Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity Pubmed:29953010 |
Veterinary immunology and immunopathology | Preactivation of neutrophils and systemic oxidative stress in dogs with hyperleptinemia Pubmed:30078593 |
Hematology | Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? Pubmed:29318945 |
International Journal of Gerontology | Aging effects on circulating adiponectin levels and the expressions of adiponectin and adiponectin receptor 1 in the brains of male rats 10.1016:j.ijge.2018.01.002 |
Oncotarget | Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120 |
J Bas Res Med Sci | Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats article-1-352-en.pdf |
Iraq Medical Journal | Adiponectin may be used as a marker in prediction of diabetic retinopathy patients JOCMS:Source |
Food Science and Technology Research | Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells Doi: 10.3136/fstr.24.903 |
Journal of Pharmacological Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783 |
Nutrients | Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet Pubmed: 30400147 |
Progress in Nutrition | Effect of fermented food consumption on biochemical parameters and adipokines levels Doi: 0.23751/pn.v20i4.7374 |
Biology of Reproduction | Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway Pubmed: 30496353 |
Nutrition in the Prevention and Treatment of Abdominal Obesity | Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig |
Evidence-Based Complementary and Alternative Medicine | BuShenKangShuai Tablet Alleviates Hepatic Steatosis via Improving Liver Adiponectin Resistance in ApoE-/-Mice |
Life Sciences | Interrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in rats Pubmed: 30826497 |
Arteriosclerosis, Thrombosis, and Vascular Biology | Cardiac Lymphatic Dysfunction Causes Drug-Eluting Stent–Induced Coronary Hyperconstricting Responses in Pigs In Vivo Pubmed: 30816801 |
Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP … Pubmed: 31003008 | |
Gene | Expression of adipokines and their receptors in adipose tissue of women with class 3 obesity with or without hypertension Pubmed: 30940525 |
中国药理学通报 | 冷暴露对高脂饮食小鼠脂肪棕色化的影响 |
Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights … |
Proceedings of the Royal Society B: Biological Sciences | Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens Pubmed: 31506054 |
Cytokine | Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus Pubmed: 31514105 |
Food & Function | Collaborative Effects of Chlorogenic Acid and Caffeine on Lipid Metabolism via AMPKα-LXRα/SREBP-1c Pathway in High-Fat Diet-Induced Obese Mice Pubmed: 31670366 |
journal of ethnopharmacology | Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model Pubmed: 31756448 |
Annals of Nutrition and Metabolism | Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity Pubmed: 31775140 |
BMC Endocrine Disorders | The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561 |
Journal of Hazardous Materials | Triphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal mice Pubmed: 31796355 |
Experimental and Clinical Endocrinology & Diabetes | HOMA-Adiponectin Closely Associates with Cardiometabolic Risk Markers in Middle-Aged Indians with Metabolic Syndrome Pubmed: 32189318 |
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Cerasus humilis cherry polyphenol reduces high-fat diet-induced obesity in C57BL/6 mice by mitigating fat deposition, inflammation, and oxidation Pubmed: 32227855 |
Nature Communications | AdipoR1/AdipoR2 dual agonist recovers nonalcoholic steatohepatitis and related fibrosis via endoplasmic reticulum-mitochondria axis Pubmed: 33199780 |
Metabolites | Adipocytokines and Metabolic Syndrome in Patients with Schizophrenia Pubmed: 33066473 |
Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity Pubmed: 33027789 |
aging | Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats Pubmed: 32970613 |
Diabetes Metab Syndr Obes | Serum ZAG and Adiponectin Levels Were Closely Related to Obesity and the Metabolically Abnormal Phenotype in Chinese Population Pubmed: 32904655 |
DIABETOLOGIA | Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via … Pubmed: 32880686 |
J Endocrinol Invest | Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … Pubmed: 32816247 |
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity Pubmed: 32633953 |
J Endocrinol Invest | Activation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic rats Pubmed: 32588381 |
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY | The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients Pubmed: 32569480 |
Diabetes | Exercise-Induced Increases in Insulin Sensitivity After Bariatric surgery are Mediated by Muscle Extracellular Matrix Remodeling Pubmed: 32409493 |
Journal of Cellular and Molecular Medicine | Cooperative effects of galanin and leptin on alleviation of insulin resistance in adipose tissue of diabetic rats Pubmed: 32395890 |
The Egyptian Journal of Chest Diseases and Tuberculosis | Study of serum adiponectin and resistin in patients with obstructive sleep apnea |
Biosciences Biotechnology Research Asia | Association of+ 10211T/G (Rs17846866) Variant of Adiponectin Gene With Type 2 Diabetes Mellitus |
Computational Biology and Chemistry | In-silico therapeutic investigations of arjunic acid and arjungenin as an FXR agonist and validation in 3T3-L1 adipocytes |
Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren 33575861 |
Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren 33834274 |
Cell Metab | TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease 34107313 |
Biomedical and Environmental Sciences | Decreased Plasma MANF Levels are Associated with Type 2 Diabetes 33766221 |
International Journal of Endocrinology | Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡ 34035808 |
International Journal of Pharmacy and Biological Sciences | Anti-Obesity Efficacy of Selected Plants Against Progesterone Induced Obesity |
Journal of Functional Foods | Smilax china L. polyphenols alleviates obesity and inflammation by modulating gut microbiota in high fat/high sucrose diet-fed C57BL/6J mice |
Indian Journal of Forensic Medicine & Toxicology | Altered Serum Marker of Adipokines Profile in Breast Cancer Women |
PPAR Research | Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment … 34306045 |
Br J Nutr | The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study 34348809 |
Phytomedicine | Smilax china L. flavonoid alleviates HFHS-induced inflammation by regulating the gut-liver axis in mice 34561124 |
Biofactors | Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat 34767670 |
Kaohsiung J Med Sci | Associations of imbalance of intestinal flora with severity of disease, inflammatory factors, adiponectin, and vascular endothelial function of hypertension patients 34672426 |
Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313 |
Animals | Effects of Obesity on Adiponectin System Skin Expression in Dogs: A Comparative Study 34438765 |
Cell Death & Disease | Hepatic deficiency of selenoprotein S exacerbates hepatic steatosis and insulin resistance Pubmed:35347118 |
Diabetes Metabolic Syndrome and Obesity-Targets and Therapy | Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in India Pubmed:35717896 |
BioMed Research International | Adiponectin Levels Are Associated with White Matter Lesions (WMLs) and Cognitive Impairment Pubmed:35087911 |
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients | |
Scientific Reports | SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions. |
Catalog No. | Related products for research use of Chicken (Gallus) Organism species | Applications (RESEARCH USE ONLY!) |
RPA605Ga01 | Recombinant Adiponectin (ADPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA605Ga01 | Active Adiponectin (ADPN) | Cell culture; Activity Assays. |
PAA605Ga01 | Polyclonal Antibody to Adiponectin (ADPN) | WB; IHC; ICC; IP. |
MAA605Ga21 | Monoclonal Antibody to Adiponectin (ADPN) | WB; IHC; ICC; IP. |
SEA605Ga | ELISA Kit for Adiponectin (ADPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA605Ga | CLIA Kit for Adiponectin (ADPN) | Chemiluminescent immunoassay for Antigen Detection. |
LMA605Ga | Multiplex Assay Kit for Adiponectin (ADPN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |