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Active Tissue Factor (TF)

CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III

ACTIVITY TEST

TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Peptides Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive rats PubMed: 18625276
Canadian Journal of Physiology and Pharmacology Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factor Nrcresearchpress: y10-115
International immunopharmacology Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in rats Pubmed: 23538027
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy Pubmed:25505239
Hippokratia Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway Inhibitor PubMed: 20011089
shock Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock PubMed: 25423126
Perfusion Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing PubMed: 26243277
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors PubMed: 25546502
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice Pubmed:26757699
Blood Cells, Inflammation and Infection Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury pubmed:29202212
Thrombosis andHaemostasis Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome Pubmed: 29202212
Journal of Thrombosis and Haemostasis Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats Pubmed: 31755229
Mediators Inflamm Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB … Pubmed: 32587471
International Journal of Clinical Practice Effect of Glucocorticoid Administration in Intravenous Pulses on Selected Parameters of the Coagulation System Pubmed:35685509
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA524Ra01 Recombinant Tissue Factor (TF) Positive Control; Immunogen; SDS-PAGE; WB.
APA524Ra01 Active Tissue Factor (TF) Cell culture; Activity Assays.
PAA524Ra01 Polyclonal Antibody to Tissue Factor (TF) WB; IHC; ICC; IP.
LAA524Ra81 FITC-Linked Polyclonal Antibody to Tissue Factor (TF) WB; IHC; ICC; IF.
LAA524Ra71 Biotin-Linked Polyclonal Antibody to Tissue Factor (TF) WB; IHC; ICC.
MAA524Ra21 Monoclonal Antibody to Tissue Factor (TF) WB; IHC; ICC; IP.
SEA524Ra ELISA Kit for Tissue Factor (TF) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA524Ra Multiplex Assay Kit for Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.