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Active Paraoxonase 1 (PON1)

ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1

ACTIVITY TEST

Paraoxonase 1 (PON1) is responsible for hydrolysing organophosphate pesticides and nerve gasses. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). Besides, Clusterin (CLU) has been identified as an interactor of PON1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON1 and recombinant human CLU. Briefly, PON1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CLU-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PON1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of PON1 and CLU was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Digestive Diseases and Sciences Fatty Liver Is Associated with Recurrent Bacterial Infections Independent of Metabolic Syndrome PubMed: 20205513
Clinica Chimica Acta Persistent elevation of paraoxonase-1 specific enzyme activity after weight reduction in obese non-diabetic men with metabolic syndrome PubMed: 21704607
Journal of Laparoendoscopic & Advanced Surgical Techniques Combined Serum Proteomic and Metabonomic Profiling After Laparoscopic Sleeve Gastrectomy in Children and Adolescents Wiley: source
PLoS One. High density lipoprotein structural changes and drug response in lipidomic profiles following the long-term fenofibrate therapy in the FIELD substudy PubMed: 21887280
Atherosclerosis Macrophage cholesterol efflux to plasma and HDL in subjects with low and high homocysteine levels: a FIELD substudy PubMed: 21696738
Lipids in Health and Disease Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome PubMed: PMC3480889
British Journal of Nutrition Extra-virgin olive oil consumption reduces the age-related decrease in HDL and paraoxonase 1 anti-inflammatory activities Pubmed: 23510814
Diabetes & Metabolic Syndrome Paraoxonase 1: A better atherosclerotic risk predictor than HDL in type 2 diabetes mellitus Pubmed: 23680251
Redox Report Paraoxonase responses to exercise and niacin therapy in men with metabolic syndrome Pubmed:25180827
Toxicol Ind Health. Influence of α-tocopherol and α-lipoic acid on bisphenol-A-induced oxidative damage in liver and ovarian tissue of rats Pubmed:25548375
RETOS. Nuevas Tendencias en Educación Física, Deporte y Recreación A single session of aerobic exercise influences paraoxonase 1 activity and concentration Articulo: 345738764042
PLoS One Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with Cirrhosis PubMed: 25789864
Renal Failure Association of lipid profile with serum PON1 concentration in patients with chronic kidney disease Pubmed:26888625
Pharmacological reports The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder science:article
Clinical and Experimental Hypertension Association of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertension pubmed:27668323
Diabetes & Metabolic Syndrome: Clinical Research & Reviews Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot Study in North Indian population pubmed:29254890
Diabetes/Metabolism Research and Reviews A Case-control Study: The Association of Serum Paraoxonase 1 Activity and Concentration with the Development of Type 2 Diabetes Mellitus pubmed:29156090
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858
Pharmacological Reports The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. pubmed:28623707
American Journal of Food Science and Health Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar Rats ISSN: 2381-7224
Metabolic brain disease Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levels pubmed:28808839
Therapeutic Apheresis And Dialysis Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney Disease Pubmed:29368426
Biological Trace Element Research Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in Rats Pubmed: 30251228
GLYCOCONJUGATE JOURNAL Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer Pubmed: 30607521
Hematology. Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails Pubmed: 30661467
Proteomics Clinical Applications Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy Pubmed: 31162828
Environment International Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures Pubmed: 31639605
Nutrition, Metabolism and Cardiovascular Diseases Phenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intake Pubmed: 31757567
Environment International Estimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteins Pubmed: 31783242
RESPIRATORY RESEARCH The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation … Pubmed: 33148285
CLINICAL RHEUMATOLOGY PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patients Pubmed: 32556934
respiratory research The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans 33407555
PLoS One Vitamin D status and systemic redox biomarkers in adults with obesity 30973911
Oxid Med Cell Longev Repetitions of Strenuous Exercise Consistently Increase Paraoxonase 1 Concentration and Activity in Plasma of Average-Trained Men 34917230
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