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Multiplex Assay Kit for Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay)
ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA243Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.2-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.067 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 393
US$ 408
US$ 431
US$ 461
US$ 491
US$ 537
US$ 605
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 80-104 | 90 |
EDTA plasma(n=5) | 79-105 | 88 |
heparin plasma(n=5) | 98-105 | 102 |
sodium citrate plasma(n=5) | 78-90 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 89-98% | 82-101% | 93-101% | 96-103% |
EDTA plasma(n=5) | 94-103% | 84-96% | 97-104% | 83-94% |
heparin plasma(n=5) | 91-105% | 83-91% | 85-98% | 96-105% |
sodium citrate plasma(n=5) | 98-105% | 96-103% | 96-105% | 89-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:PON1) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Digestive Diseases and Sciences | Fatty Liver Is Associated with Recurrent Bacterial Infections Independent of Metabolic Syndrome PubMed: 20205513 |
Clinica Chimica Acta | Persistent elevation of paraoxonase-1 specific enzyme activity after weight reduction in obese non-diabetic men with metabolic syndrome PubMed: 21704607 |
Journal of Laparoendoscopic & Advanced Surgical Techniques | Combined Serum Proteomic and Metabonomic Profiling After Laparoscopic Sleeve Gastrectomy in Children and Adolescents Wiley: source |
PLoS One. | High density lipoprotein structural changes and drug response in lipidomic profiles following the long-term fenofibrate therapy in the FIELD substudy PubMed: 21887280 |
Atherosclerosis | Macrophage cholesterol efflux to plasma and HDL in subjects with low and high homocysteine levels: a FIELD substudy PubMed: 21696738 |
Lipids in Health and Disease | Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome PubMed: PMC3480889 |
British Journal of Nutrition | Extra-virgin olive oil consumption reduces the age-related decrease in HDL and paraoxonase 1 anti-inflammatory activities Pubmed: 23510814 |
Diabetes & Metabolic Syndrome | Paraoxonase 1: A better atherosclerotic risk predictor than HDL in type 2 diabetes mellitus Pubmed: 23680251 |
Redox Report | Paraoxonase responses to exercise and niacin therapy in men with metabolic syndrome Pubmed:25180827 |
Toxicol Ind Health. | Influence of α-tocopherol and α-lipoic acid on bisphenol-A-induced oxidative damage in liver and ovarian tissue of rats Pubmed:25548375 |
RETOS. Nuevas Tendencias en Educación Física, Deporte y Recreación | A single session of aerobic exercise influences paraoxonase 1 activity and concentration Articulo: 345738764042 |
PLoS One | Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with Cirrhosis PubMed: 25789864 |
Renal Failure | Association of lipid profile with serum PON1 concentration in patients with chronic kidney disease Pubmed:26888625 |
Pharmacological reports | The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder science:article |
Clinical and Experimental Hypertension | Association of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertension pubmed:27668323 |
Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot Study in North Indian population pubmed:29254890 |
Diabetes/Metabolism Research and Reviews | A Case-control Study: The Association of Serum Paraoxonase 1 Activity and Concentration with the Development of Type 2 Diabetes Mellitus pubmed:29156090 |
Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858 |
Pharmacological Reports | The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. pubmed:28623707 |
American Journal of Food Science and Health | Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar Rats ISSN: 2381-7224 |
Metabolic brain disease | Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levels pubmed:28808839 |
Therapeutic Apheresis And Dialysis | Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney Disease Pubmed:29368426 |
Biological Trace Element Research | Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in Rats Pubmed: 30251228 |
GLYCOCONJUGATE JOURNAL | Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer Pubmed: 30607521 |
Hematology. | Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails Pubmed: 30661467 |
Proteomics Clinical Applications | Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy Pubmed: 31162828 |
Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures Pubmed: 31639605 |
Nutrition, Metabolism and Cardiovascular Diseases | Phenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intake Pubmed: 31757567 |
Environment International | Estimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteins Pubmed: 31783242 |
RESPIRATORY RESEARCH | The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation … Pubmed: 33148285 |
CLINICAL RHEUMATOLOGY | PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patients Pubmed: 32556934 |
respiratory research | The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction |
J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans 33407555 |
PLoS One | Vitamin D status and systemic redox biomarkers in adults with obesity 30973911 |
Oxid Med Cell Longev | Repetitions of Strenuous Exercise Consistently Increase Paraoxonase 1 Concentration and Activity in Plasma of Average-Trained Men 34917230 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA243Hu02 | Recombinant Paraoxonase 1 (PON1) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA243Hu01 | Recombinant Paraoxonase 1 (PON1) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA243Hu01 | Active Paraoxonase 1 (PON1) | Cell culture; Activity Assays. |
RPA243Hu03 | Recombinant Paraoxonase 1 (PON1) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA243Hu04 | Recombinant Paraoxonase 1 (PON1) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA243Hu05 | Recombinant Paraoxonase 1 (PON1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA243Hu01 | Polyclonal Antibody to Paraoxonase 1 (PON1) | WB; IHC; ICC; IP. |
PAA243Hu02 | Polyclonal Antibody to Paraoxonase 1 (PON1) | WB; IHC; ICC; IP. |
LAA243Hu71 | Biotin-Linked Polyclonal Antibody to Paraoxonase 1 (PON1) | WB; IHC; ICC. |
LAA243Hu81 | FITC-Linked Polyclonal Antibody to Paraoxonase 1 (PON1) | WB; IHC; ICC; IF. |
MAA243Hu22 | Monoclonal Antibody to Paraoxonase 1 (PON1) | WB; IHC; ICC; IP. |
SEA243Hu | ELISA Kit for Paraoxonase 1 (PON1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA243Hu | CLIA Kit for Paraoxonase 1 (PON1) | Chemiluminescent immunoassay for Antigen Detection. |
LMA243Hu | Multiplex Assay Kit for Paraoxonase 1 (PON1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA243Hu01 | ELISA Kit DIY Materials for Paraoxonase 1 (PON1) | Main materials for "Do It (ELISA Kit) Yourself". |