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Active Alpha-1-Microglobulin (a1M)

AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin

ACTIVITY TEST

Alpha-1-Microglobulin (a1M) is a small protein belonging to the superfamily of lipocalins, which is present in various body fluids such as plasma and cerebrospinal fluid. it has a role in the transportation of molecules like metals and serves as a marker for certain diseases, including kidney and liver disorders.The activity of recombinant human A1M was measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Trypsin was diluted to 50 ug/ml in the assay buffer and 25 ul different concentrations of recombinant human a1M (MW: 64 KD) was incubated with 25ul diluted trypsin at 37 ℃ for 15 minutes.Loading 50 µL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 µL of 20 µM substrate.nclude a substrate blank containing 50 µL of assay buffer and 50 µL of 20 µM substrate.Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes.The result was shown in Figure 1 and it was obvious that recombinant human a1M significantly decreased trypsin activity. The inhibition IC50 was <2.2 nM.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Diabetes Technology & Therapeutics Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 Liebert: 20100078
Journal of Pharmaceutical and Biomedical Analysis Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 PubMed: 21130593
BioMed Research International Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus PubMed: PMC3679811
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Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA217Hu61 Active Alpha-1-Microglobulin (a1M) Cell culture; Activity Assays.
APA217Hu62 Active Alpha-1-Microglobulin (a1M) Cell culture; Activity Assays.
EPA217Hu61 Eukaryotic Alpha-1-Microglobulin (a1M) Positive Control; Immunogen; SDS-PAGE; WB.
EPA217Hu62 Eukaryotic Alpha-1-Microglobulin (a1M) Positive Control; Immunogen; SDS-PAGE; WB.
RPA217Hu01 Recombinant Alpha-1-Microglobulin (a1M) Positive Control; Immunogen; SDS-PAGE; WB.
PAA217Hu01 Polyclonal Antibody to Alpha-1-Microglobulin (a1M) WB
LAA217Hu71 Biotin-Linked Polyclonal Antibody to Alpha-1-Microglobulin (a1M) WB; IHC; ICC.
MAA217Hu22 Monoclonal Antibody to Alpha-1-Microglobulin (a1M) WB; IHC; ICC; IP.
SEA217Hu ELISA Kit for Alpha-1-Microglobulin (a1M) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA217Hu CLIA Kit for Alpha-1-Microglobulin (a1M) Chemiluminescent immunoassay for Antigen Detection.
LMA217Hu Multiplex Assay Kit for Alpha-1-Microglobulin (a1M) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.