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Active Matrix Metalloproteinase 13 (MMP13)

CLG3; Collagenase 3

ACTIVITY TEST

Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. MMP13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. MMP13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. Although gelatin zymography is mainly used for the detection of the MMP2 and MMP9, it also can be used for MMP13 dection. Briefly, various concentrations of MMP13 (1000ng, 500ng, 250ng, 125ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze gelatin nearby, which was indicated by the white bands on the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant rat MMP13 was shown in figure 1.

USAGE

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression ScienceDirect: S0925443911001323
Journal of Physiology and Biochemistry Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats SpringerLink: h875w73171w2147w
FEBS Journal Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta Wiley: source
Arthritis & Rheumatism Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice Wiley: source
Gene Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors ScienceDirect: S0378111911007347
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Arthritis Rheumatol Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression. Pubmed: 24578232
Arthritis & Rheumatology Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression Onlinelibrary: art.38576
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655
Arthritis & Rheumatol Identification of α2-Macroglobulin as a Master Inhibitor of Cartilage-Degrading Factors That Attenuates the Progression of Posttraumatic Osteoarthritis Pubmed:24578232
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261
Cellular Physiology and Biochemistry Inverse Regulation of Early and Late Chondrogenic Differentiation by Oxygen Tension Provides Cues for Stem Cell-Based Cartilage Tissue Engineering Pubmed:25632940
Osteoarthritis and Cartilage Depletion of gangliosides enhances cartilage degradation in mice Sciencedirect:S1063458413010364
Biol Res The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310
PLoS One Interleukin-1 Receptor Antagonist Has a Novel Function in the Regulation of Matrix Metalloproteinase-13 Expression PubMed: 26474296
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496
Scientific Reports Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds pubmed:27821864
International Journal of Contemporary Medical Research Matrix Metalloproteinase-13 (MMP-13): A Novel Tumor Marker for Diagnosis of Breast Carcinoma uploads:7
Scientific Reports Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption. pubmed:27640363
Photodermatol Photoimmunol Photomed. Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice pubmed:27995657
Molecular Medicine Reports Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells pubmed:28447732
bmc musculoskeletal disorders Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. pubmed:28187731
Journal of Cellular Physiology Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models pubmed:27739057
BMC Musculoskeletal Disorders Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture pubmed:29149841
Journal of Applied Animal Research Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis 10.108:09712119.2017.1335208
Alexandria Journal of Medicine L-Carnitine ameliorates knee lesions in mono-iodoacetate induced osteoarthritis in rats 10.1016:j.ajme.2016.03.002
National Journal of Integrated Research in Medicine Matrix Metalloproteinase-13 Can Be Used As an Independent Diagnostic and Prognostic Marker for Breast Carcinoma. AN=123116960
Molecular Medicine Reports Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits Pubmed:29257344
Journal of Applied Animal Research  Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis 10.1080:09712119.2017.1335208
Osteoarthritis Cartilage Coordinated existence of multiple gangliosides is required for cartilage metabolism Pubmed: 30471358
PLoS One Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis Pubmed: 30939166
Pathobiology Suppressed Expression but Not Activity of Collagenases MMP-1 and MMP-13 in Human Renal Carcinoma Pubmed: 31129674
Bone DAla2GIP antagonizes H2O2-induced chondrocyte apoptosis and inflammatory factor secretion Pubmed: 31283994
Food & Function A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese …
JOURNAL OF CELLULAR BIOCHEMISTRY Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes Pubmed: 32162384
Bone Research Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis Pubmed: 32047705
Scientific Reports Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722
Nutrients Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial Pubmed: 31888255
Nature Communications Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13 Pubmed: 32807790
Molecular Medicine Reports MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression Pubmed: 32705199
RESEARCH SQUARE Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs
Hyaluronic Acid versus Alpha-2-Macroglobulin Intra-Articular Injections for Amelioration of Knee Posttraumatic Osteoarthritis: A Rat Model
Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs
Food Funct Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice 33890588
Nutrients Metabolic and Anti-Inflammatory Protective Properties of Human Enriched Serum Following Artichoke Leaf Extract Absorption: Results from an Innovative Ex Vivo … 34444810
Sci Rep Liraglutide, a glucagon-like peptide 1 receptor agonist, exerts analgesic, anti-inflammatory and anti-degradative actions in osteoarthritis Pubmed:35091584
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
EPA099Ra61 Eukaryotic Matrix Metalloproteinase 13 (MMP13) Positive Control; Immunogen; SDS-PAGE; WB.
RPA099Ra01 Recombinant Matrix Metalloproteinase 13 (MMP13) Positive Control; Immunogen; SDS-PAGE; WB.
RPA099Ra02 Recombinant Matrix Metalloproteinase 13 (MMP13) Positive Control; Immunogen; SDS-PAGE; WB.
APA099Ra61 Active Matrix Metalloproteinase 13 (MMP13) Cell culture; Activity Assays.
PAA099Ra01 Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13) WB; IHC; ICC; IP.
PAA099Ra02 Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13) WB; IHC; ICC; IP.
LAA099Ra71 Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13) WB; IHC; ICC.
SEA099Ra ELISA Kit for Matrix Metalloproteinase 13 (MMP13) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA099Ra CLIA Kit for Matrix Metalloproteinase 13 (MMP13) Chemiluminescent immunoassay for Antigen Detection.
LMA099Ra Multiplex Assay Kit for Matrix Metalloproteinase 13 (MMP13) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.