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Active Interleukin 15 (IL15)

ACTIVITY TEST

Interleukin 15 (IL15), encoded by the IL15 gene in humans, is a pleiotropic cytokine of the common gamma-chain family. It plays critical roles in immune regulation, especially in maintaining the survival, proliferation, and activation of T cells, natural killer (NK) cells, and dendritic cells. Produced mainly by monocytes, macrophages, and epithelial cells, IL15 exerts its biological effects via a unique "trans-presentation" mechanism, often relying on binding to its receptor. IL15 is linked to immune responses against infections and tumors, and its dysregulation is associated with autoimmune diseases and inflammatory disorders. IL15 binds specifically to Interleukin 15 Receptor alpha (IL15Ra, encoded by IL15RA), forming a complex to initiate downstream signaling.Thus a functional ELISA assay was conducted to detect the interaction of recombinant musculus IL15 and recombinant human IL15Ra. Briefly, IL15 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL15Ra-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL15 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant musculus IL15 and recombinant human IL15Ra was shown in Figure 1, the EC50 for this effect is 0.21561µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Endocrine Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training Pubmed: 24006180
Endocrine Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype Pubmed:25522723
Med Sci Moni miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice PubMed: 26087886
Exercise physiology Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle Dspace: Handle
Biotechnology Letters Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice s10529-017-2342-1
Journal of Controlled Release Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. pubmed:28408199
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