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Active Insulin Like Growth Factor Binding Protein 2 (IGFBP2)
IBP2; IGF-BP53
- Product No.APA053Mu61
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point7.3
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 288
US$ 720
US$ 1440
US$ 4320
US$ 10800
For more details, please contact local distributors!
ACTIVITY TEST
Insulin Like Growth Factor Binding Protein 2 (IGFBP2) is a key member of the IGFBP family that modulates the bioavailability and activity of insulin-like growth factors (IGF-I and IGF-II). Primarily secreted by the liver, IGFBP2 binds IGFs with high affinity, prolonging their half-life while regulating their interaction with IGF receptors. Beyond its classical IGF-regulatory role, IGFBP2 exhibits IGF-independent functions in cell migration, apoptosis, and angiogenesis through interactions with integrins and extracellular matrix components. To detect the activity of recombinant IGFBP2, a functional ELISA assay was performed to evaluate the interaction between recombinant mouse IGFBP2 and recombinant human Insulin Like Growth Factor 1 (IGF1).Briefly, IGFBP2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IGF1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IGFBP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse IGFBP2 and recombinant human IGF1 was shown in Figure 1, the EC50 for this effect is 0.017 µg/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| British journal of cancer | A combined biomarker panel shows improved sensitivity for the early detection of ovarian cancer allowing the identification of the most aggressive type II tumours. pubmed:28664912 |
| Experimental and Therapeutic Medicine | Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D‑LC‑MS/MS Doi: 10.3892/etm.2018.6818 |
| Nutrition and Cancer | Serum IGFBP2 Level Is a New Candidate Biomarker of Severe Malnutrition in Advanced Lung Cancer Pubmed: 32286106 |