≡

Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)

CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor

ACTIVITY TEST

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein that functions as a cytokine — it is a white blood cell growth factor. GM-CSF also has some effects on mature cells of the immune system. These include, for example, inhibiting neutrophil migration and causing an alteration of the receptors expressed on the cells surface. Besides,the proliferation of TF-1 cell line GM-CSF basic-dependent, thus, the activity of GM-CSF is usually measured by a cell proliferation assay using TF-1 cells. TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant sheep GM-CSF. After incubated for 2 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 ℃. Proliferation of TF-1 cells after incubation with GM-CSF for 2 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant sheep GM-CSF for 2 days. The result was shown in Figure 2. It was obvious that GM-CSF significantly increased cell viability of TF-1 cells. The ED50 is 13.9 ng/ml.

Figure 2. Cell proliferation of TF-1 cells after stimulated with GM-CSF.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Toxicology and Environmental Health Effects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic System PubMed: 20391130
5 Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trial Pubmed:24525316
Archives of Virology Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China Pubmed:29305646
BioMed Research International Activated Macrophages of Monocytic Origin Predominantly Express Proinflammatory Cytokine Genes, Whereas Kupffer Cells Predominantly Express Anti …
bulletin of experimental biology and medicine Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin Pubmed: 31183654
Exp Mol Pathol The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­ 33285209
Vaccine Co-delivery of PSMA antigen epitope and mGM-CSF with a cholera toxin-like chimeric protein suppressed prostate tumor growth via activating dendritic cells and?¡­ 33612342
Catalog No. Related products for research use of Ovis aries; Ovine (Sheep) Organism species Applications (RESEARCH USE ONLY!)
APA045Ov01 Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Cell culture; Activity Assays.
RPA045Ov01 Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Positive Control; Immunogen; SDS-PAGE; WB.