ELISA Kit for Fibroblast Growth Factor 15 (FGF15)
- Product No.CEL154Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.5-200pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.2pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 15 (FGF15).
No significant cross-reactivity or interference between Fibroblast Growth Factor 15 (FGF15) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 15 (FGF15) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 15 (FGF15) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 15 (FGF15) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 15 (FGF15) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 15 (FGF15) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Endocrinology.||Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21 PubMed: 25774553|
|Inflammatory Bowel Diseases||Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammation pubmed:27580383|
|J Lipid Res.||Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis. pubmed:28130274|
|BMC Endocrine Disorders||The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance 10.1186/s12902-017-0211-5|
|Journal of Physiology and Biochemistry||The ileal FGF15/19 to hepatic FGFR4 axis regulates liver regeneration after partial hepatectomy in mice Pubmed:29468415|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPL154Ra01||Recombinant Fibroblast Growth Factor 15 (FGF15)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAL154Ra01||Polyclonal Antibody to Fibroblast Growth Factor 15 (FGF15)||WB; IHC; ICC; IP.|
|MAL154Ra21||Monoclonal Antibody to Fibroblast Growth Factor 15 (FGF15)||WB; IHC; ICC; IP.|
|WEL154Ra||Wide-range ELISA Kit for Fibroblast Growth Factor 15 (FGF15)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEL154Ra||ELISA Kit for Fibroblast Growth Factor 15 (FGF15)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEL154Ra||ELISA Kit for Fibroblast Growth Factor 15 (FGF15)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSL154Ra01||ELISA Kit DIY Materials for Fibroblast Growth Factor 15 (FGF15)||Main materials for "Do It(ELISA Kit) Yourself"|